CpG methylation in cell-free Epstein-Barr virus DNA in patients with EBV-hodgkin lymphoma

Meir Shamay, Jennifer A. Kanakry, John S.W. Low, Netanel A. Horowitz, Guy Journo, Anuj Ahuja, Yonatan Eran, Elinor Barzilai, Eldad J. Dann, Jennifer Stone, Wan Lu Woo, Wen Son Hsieh, Rena R. Xian, Richard F. Ambinder

Research output: Contribution to journalArticlepeer-review

Abstract

Epstein-Barr virus (EBV) is associated with a variety of tumors and nonmalignant conditions. Latent EBV genomes in cells, including tumor cells, are often CpG methylated, whereas virion DNA is not CpG methylated. We demonstrate that methyl CpG binding magnetic beads can be used to fractionate among sources of EBV DNA (DNA extracted from laboratory-purified virions vs DNA extracted from latently infected cell lines). We then applied the technique to plasma specimens and showed that this technique can distinguish EBV DNA from patients with EBV-associated tumors (nasopharyngeal carcinoma, Hodgkin lymphoma) and viral DNA from patients without EBV-associated tumors, including immunocompromised patients and patients with EBV(2) Hodgkin lymphoma.

Original languageEnglish (US)
Pages (from-to)1624-1627
Number of pages4
JournalBlood Advances
Volume4
Issue number8
DOIs
StatePublished - Apr 28 2020

ASJC Scopus subject areas

  • Hematology

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