CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1

Angela H. Ting, Kam Wing Jair, Hiromu Suzuki, Ray Whay Chiu Yen, Stephen B. Baylin, Kornel E. Schuebel

Research output: Contribution to journalArticle


The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.

Original languageEnglish (US)
Pages (from-to)582-584
Number of pages3
JournalNature genetics
Issue number6
StatePublished - Jun 1 2004


ASJC Scopus subject areas

  • Genetics

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