CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1

Angela H. Ting, Kam Wing Jair, Hiromu Suzuki, Ray Whay Chiu Yen, Stephen B. Baylin, Kornel E. Schuebel

Research output: Contribution to journalArticle

Abstract

The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.

Original languageEnglish (US)
Pages (from-to)582-584
Number of pages3
JournalNature genetics
Volume36
Issue number6
DOIs
StatePublished - Jun 1 2004

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ASJC Scopus subject areas

  • Genetics

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