COX-2 and PGE2 signaling is essential for the regulation of IDO expression by curcumin in murine bone marrow-derived dendritic cells

In Duk Jung, Young Il Jeong, Chang Min Lee, Kyung Tae Noh, Soo Kyung Jeong, Sung Hak Chun, Oksoon Hong Choi, Won Sun Park, Jin Han, Yong Kyoo Shin, Han Wool Kim, Cheol Heui Yun, Yeong Min Park

Research output: Contribution to journalArticle

Abstract

Indoleamine 2,3-dioxygenase (IDO), a key enzyme that catalyzes the initial, rate-limiting step in tryptophan degradation, is expressed in dendritic cells (DCs) which are stimulated by lipopolysaccharide (LPS) or interferons. In this study we show that curcumin inhibits IDO expression in vitro and in vivo in DCs, leading to the suppression of LPS-induced DC maturation. The effect of curcumin relative to LPS is not limited to the above, as it also enhances LPS-induced expression of cyclooxygenase (COX)-2 and production of prostaglandin E2 (PGE2). Additionally, PGE2 diminished the LPS-induced IDO expression in DCs, thereby contributing to the inhibition of expression of the surface molecules (CD80, CD86 and MHC class I) and the production of the proinflammatory cytokines (IL-12 p70 and TNF-α) by LPS stimulation. Under our experimental conditions, curcumin plays an immunomodulatory role by downregulating IDO expression via a COX-2/PGE2-dependant pathway, thus impacting DC maturation in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)760-768
Number of pages9
JournalInternational Immunopharmacology
Volume10
Issue number7
DOIs
StatePublished - Jul 2010

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Indoleamine-Pyrrole 2,3,-Dioxygenase
Curcumin
Cyclooxygenase 2
Dinoprostone
Dendritic Cells
Lipopolysaccharides
Bone Marrow
Interleukin-12
Tryptophan
Interferons
Down-Regulation
Cytokines
Enzymes

Keywords

  • Curcumin
  • Cyclooxygenase
  • Dendritic cells
  • Indoleamine 2,3-dioxygenase

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

COX-2 and PGE2 signaling is essential for the regulation of IDO expression by curcumin in murine bone marrow-derived dendritic cells. / Jung, In Duk; Jeong, Young Il; Lee, Chang Min; Noh, Kyung Tae; Jeong, Soo Kyung; Chun, Sung Hak; Choi, Oksoon Hong; Park, Won Sun; Han, Jin; Shin, Yong Kyoo; Kim, Han Wool; Yun, Cheol Heui; Park, Yeong Min.

In: International Immunopharmacology, Vol. 10, No. 7, 07.2010, p. 760-768.

Research output: Contribution to journalArticle

Jung, ID, Jeong, YI, Lee, CM, Noh, KT, Jeong, SK, Chun, SH, Choi, OH, Park, WS, Han, J, Shin, YK, Kim, HW, Yun, CH & Park, YM 2010, 'COX-2 and PGE2 signaling is essential for the regulation of IDO expression by curcumin in murine bone marrow-derived dendritic cells', International Immunopharmacology, vol. 10, no. 7, pp. 760-768. https://doi.org/10.1016/j.intimp.2010.04.006
Jung, In Duk ; Jeong, Young Il ; Lee, Chang Min ; Noh, Kyung Tae ; Jeong, Soo Kyung ; Chun, Sung Hak ; Choi, Oksoon Hong ; Park, Won Sun ; Han, Jin ; Shin, Yong Kyoo ; Kim, Han Wool ; Yun, Cheol Heui ; Park, Yeong Min. / COX-2 and PGE2 signaling is essential for the regulation of IDO expression by curcumin in murine bone marrow-derived dendritic cells. In: International Immunopharmacology. 2010 ; Vol. 10, No. 7. pp. 760-768.
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AU - Choi, Oksoon Hong

AU - Park, Won Sun

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AU - Park, Yeong Min

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