Abstract
Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methylation profiling, but the associated high sequencing costs continue to limit its widespread application. We used several high-coverage reference data sets to experimentally determine minimal sequencing requirements. We present data-derived recommendations for minimum sequencing depth for WGBS libraries, highlight what is gained with increasing coverage and discuss the trade-off between sequencing depth and number of assayed replicates.
Original language | English (US) |
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Pages (from-to) | 230-232 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 12 |
Issue number | 3 |
DOIs | |
State | Published - Feb 26 2015 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology