Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing

Michael J. Ziller, Kasper Daniel Hansen, Alexander Meissner, Martin J. Aryee

Research output: Contribution to journalArticle

Abstract

Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methylation profiling, but the associated high sequencing costs continue to limit its widespread application. We used several high-coverage reference data sets to experimentally determine minimal sequencing requirements. We present data-derived recommendations for minimum sequencing depth for WGBS libraries, highlight what is gained with increasing coverage and discuss the trade-off between sequencing depth and number of assayed replicates.

Original languageEnglish (US)
Pages (from-to)230-232
Number of pages3
JournalNature Methods
Volume12
Issue number3
DOIs
StatePublished - Feb 26 2015

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Methylation
Genes
Genome
DNA Fingerprinting
DNA Methylation
Libraries
Costs and Cost Analysis
hydrogen sulfite
Costs

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing. / Ziller, Michael J.; Hansen, Kasper Daniel; Meissner, Alexander; Aryee, Martin J.

In: Nature Methods, Vol. 12, No. 3, 26.02.2015, p. 230-232.

Research output: Contribution to journalArticle

Ziller, Michael J. ; Hansen, Kasper Daniel ; Meissner, Alexander ; Aryee, Martin J. / Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing. In: Nature Methods. 2015 ; Vol. 12, No. 3. pp. 230-232.
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