Covalently dye-linked, surface-controlled, and bioconjugated organically modified silica nanoparticles as targeted probes for optical imaging

Rajiv Kumar, Indrajit Roy, Tymish Y. Ohulchanskyy, Lalit N. Goswami, Adela C. Bonoiu, Earl J. Bergey, Kenneth M. Tramposch, Anirban Maitra, Paras N. Prasad

Research output: Contribution to journalArticle

Abstract

In this paper we report the synthesis and characterization of organically modified silica (ORMOSIL) nanoparticles, covalently incorporating the fluorophore rhodamine-B, and surface-functionalized with a variety of active groups. The synthesized nanoparticles are of ultralow size (diameter ∼20 nm), highly monodispersed, stable in aqueous suspension, and retain the optical properties of the incorporated fluorophore. The surface of the nanoparticles can be functionalized with a variety of active groups such as hydroxyl, thiol, amine, and carboxyl. The carboxyl groups on the surface were used to conjugate with various bioactive molecules such as transferrin, as well as monoclonal antibodies such as anti-claudin 4 and anti-mesothelin, for targeted delivery to pancreatic cancer cell lines. In vitro experiments have revealed that the cellular uptake of these bioconjugated (targeted) nanoporticles is significantly higher than that of the nonconjugated ones. The ease of surface functionalization and incorporation of a variety of biotargeting molecules, combined with their observed noncytotoxity, makes these fluorescent ORMOSIL nanoparticles potential candidates as efficient probes for optical bioimaging, both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)449-456
Number of pages8
JournalACS Nano
Volume2
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • Bioconjugation
  • Cellular uptake
  • Optical imaging
  • ORMOSIL nanoparticles
  • Pancreatic cancer

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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  • Cite this

    Kumar, R., Roy, I., Ohulchanskyy, T. Y., Goswami, L. N., Bonoiu, A. C., Bergey, E. J., Tramposch, K. M., Maitra, A., & Prasad, P. N. (2008). Covalently dye-linked, surface-controlled, and bioconjugated organically modified silica nanoparticles as targeted probes for optical imaging. ACS Nano, 2(3), 449-456. https://doi.org/10.1021/nn700370b