TY - JOUR
T1 - Coupling traction force patterns and actomyosin wave dynamics reveals mechanics of cell motion
AU - Ghabache, Elisabeth
AU - Cao, Yuansheng
AU - Miao, Yuchuan
AU - Groisman, Alex
AU - Devreotes, Peter N.
AU - Rappel, Wouter Jan
N1 - Funding Information:
We thank Dr. Sangyoon Han for many helpful discussions. This work was supported by HFSP number LT000371/2017‐C (E.G.), National Institutes of Health Grant R35GM118177 (to P.N.D.), National Science Foundation under grants PHY‐1707637 and DMS‐1953469, and Department of Defense Award W81XWH‐20‐1‐0444 Program BC190068 (W.‐J.R.).
Publisher Copyright:
© 2021 The Authors. Published under the terms of the CC BY 4.0 license
PY - 2021/12
Y1 - 2021/12
N2 - Motile cells can use and switch between different modes of migration. Here, we use traction force microscopy and fluorescent labeling of actin and myosin to quantify and correlate traction force patterns and cytoskeletal distributions in Dictyostelium discoideum cells that move and switch between keratocyte-like fan-shaped, oscillatory, and amoeboid modes. We find that the wave dynamics of the cytoskeletal components critically determine the traction force pattern, cell morphology, and migration mode. Furthermore, we find that fan-shaped cells can exhibit two different propulsion mechanisms, each with a distinct traction force pattern. Finally, the traction force patterns can be recapitulated using a computational model, which uses the experimentally determined spatiotemporal distributions of actin and myosin forces and a viscous cytoskeletal network. Our results suggest that cell motion can be generated by friction between the flow of this network and the substrate.
AB - Motile cells can use and switch between different modes of migration. Here, we use traction force microscopy and fluorescent labeling of actin and myosin to quantify and correlate traction force patterns and cytoskeletal distributions in Dictyostelium discoideum cells that move and switch between keratocyte-like fan-shaped, oscillatory, and amoeboid modes. We find that the wave dynamics of the cytoskeletal components critically determine the traction force pattern, cell morphology, and migration mode. Furthermore, we find that fan-shaped cells can exhibit two different propulsion mechanisms, each with a distinct traction force pattern. Finally, the traction force patterns can be recapitulated using a computational model, which uses the experimentally determined spatiotemporal distributions of actin and myosin forces and a viscous cytoskeletal network. Our results suggest that cell motion can be generated by friction between the flow of this network and the substrate.
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U2 - 10.15252/msb.202110505
DO - 10.15252/msb.202110505
M3 - Article
C2 - 34898015
AN - SCOPUS:85121875861
SN - 1744-4292
VL - 17
JO - Molecular systems biology
JF - Molecular systems biology
IS - 12
M1 - e10505
ER -