@article{15237198e9be4135b581a96eecac0328,
title = "Coupling of mGluR/Homer and PSD-95 complexes by the Shank family of postsynaptic density proteins",
abstract = "Shank is a recently described family of postsynaptic proteins that function as part of the NMDA receptor-associated PSD-95 complex (Naisbitt et al., 1999 [this issue of Neuron]). Here, we report that Shank proteins also bind to Homer. Homer proteins form multivalent complexes that bind proline- rich motifs in group 1 metabotropic glutamate receptors and inositol trisphosphate receptors, thereby coupling these receptors in a signaling complex. A single Homer-binding site is identified in Shank, and Shank and Homer coimmunoprecipitate from brain and colocalize at postsynaptic densities. Moreover, Shank clusters mGluR5 in heterologous cells in the presence of Homer and mediates the coclustering of Homer with PSD-95/GKAP. Thus, Shank may cross-link Homer and PSD-95 complexes in the PSD and play a role in the signaling mechanisms of both mGluRs and NMDA receptors.",
author = "Tu, {Jian Cheng} and Bo Xiao and Scott Naisbitt and Yuan, {Joseph P.} and Petralia, {Ronald S.} and Paul Brakeman and Andrew Doan and Aakalu, {Vinay K.} and Lanahan, {Anthony A.} and Morgan Sheng and Worley, {Paul F.}",
note = "Funding Information: We thank Dr. Y.-X. Wang for assistance in the immunogold immunocytochemistry, Mutsuo Nuriya for assistance with GST pulldown assays, and Maria Papapavlou for laboratory assistance. This work was supported by grants to P. W. from the National Institute on Drug Abuse (DA10309 and DA11742), the National Institute of Mental Health (KO2 MH01152), the National Institute on Aging (AG09219), and the National Association for Research on Schizophrenia and Affective Disorders and by a grant to M. S. from the National Institute on Neurological Diseases and Stroke (NS35050). M. S. is an Assistant Investigator of the Howard Hughes Medical Institute. R. S. P. was supported by the National Insitute on Deafness and Other Communication Disorders Intramural Program. We thank Richard Huganir for mGluR5 antibodies.",
year = "1999",
month = jul,
doi = "10.1016/S0896-6273(00)80810-7",
language = "English (US)",
volume = "23",
pages = "583--592",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",
}