Coupling between H+ entry and ATP formation in Escherichia coli

Peter C. Maloney

Research output: Contribution to journalArticlepeer-review

Abstract

ATP synthesis driven by a pH gradient was studied in wild type Escherichia coli and in a mutant lacking functional membrane bound Ca++, Mg++-stimulated adenosine 5′-triphosphatase (BF0F1; EC 3.6.1.3). In the wild type, ATP synthesis required the presence of mobile ions other than H+; ATP synthesis was not observed in the mutant. Direct measurement of internal pH also showed that when ATP synthesis occurred in the wild type, acidification of the cell was more rapid than in the mutant. These observations provide direct evidence in support of the idea that BF0F1 catalyzes an obligatory coupling between H+ entry and ATP synthesis.

Original languageEnglish (US)
Pages (from-to)1496-1501
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume83
Issue number4
DOIs
StatePublished - Aug 29 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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