TY - JOUR
T1 - Cotransplantation of human mesenchymal stem cells enhances human myelopoiesis and megakaryocytopoiesis in NOD/SCID mice
AU - Angelopoulou, Maria
AU - Novelli, Enrico
AU - Grove, Joanna E.
AU - Rinder, Henry M.
AU - Civin, Curt
AU - Cheng, Linzhao
AU - Krause, Diane S.
N1 - Funding Information:
This research was supported by NIH grants HK69207 and CA70970, and a Leukemia Lymphoma Society Translational Research Award.
PY - 2003/5/1
Y1 - 2003/5/1
N2 - Objective. For approximately 5% of autologous transplant recipients and a higher proportion of allogeneic transplant recipients, low level and delayed platelet engraftment is an ongoing problem. Mesenchymal stem cells (MSC), which can be derived from bone marrow as well as other organs, are capable of differentiation into multiple cell types and also support hematopoiesis in vitro. Because cotransplantation of marrow-derived stromal cells has been shown to enhance engraftment of human hematopoietic stem cells, we hypothesized that cotransplantation of MSC could enhance platelet and myeloid cell development. Materials and Methods. We tested this hypothesis by transplantation of CD34-selected mobilized human peripheral blood stem cells (PBSC) into sublethally irradiated NOD/SCID mice with or without culture-expanded human MSC and evaluated human myeloid, lymphoid, and megakaryocytic engraftment with flow cytometry and in vitro cultures. Results. We find that MSC cotransplantation enhances human cell engraftment when a limiting dose (<1×106) of CD34 cells is administered. This enhancement is characterized by a shift in the differentiation of human cells from predominantly B lymphocytes to predominantly CD13+, CD14+, and CD33+ myeloid cells with a corresponding increase in myeloid CFU in the marrow. Megakaryocytopoiesis is enhanced by MSC cotransplantation as assessed by an increase in both marrow CFU-MK and circulating human platelets. In contrast, MSC do not affect the percentage of human bone marrow cells that expresses CD34+. Conclusions. Cotransplantation of human mesenchymal stem cells with CD34+-selected hematopoietic stem cells enhances myelopoiesis and megakaryocytopoiesis.
AB - Objective. For approximately 5% of autologous transplant recipients and a higher proportion of allogeneic transplant recipients, low level and delayed platelet engraftment is an ongoing problem. Mesenchymal stem cells (MSC), which can be derived from bone marrow as well as other organs, are capable of differentiation into multiple cell types and also support hematopoiesis in vitro. Because cotransplantation of marrow-derived stromal cells has been shown to enhance engraftment of human hematopoietic stem cells, we hypothesized that cotransplantation of MSC could enhance platelet and myeloid cell development. Materials and Methods. We tested this hypothesis by transplantation of CD34-selected mobilized human peripheral blood stem cells (PBSC) into sublethally irradiated NOD/SCID mice with or without culture-expanded human MSC and evaluated human myeloid, lymphoid, and megakaryocytic engraftment with flow cytometry and in vitro cultures. Results. We find that MSC cotransplantation enhances human cell engraftment when a limiting dose (<1×106) of CD34 cells is administered. This enhancement is characterized by a shift in the differentiation of human cells from predominantly B lymphocytes to predominantly CD13+, CD14+, and CD33+ myeloid cells with a corresponding increase in myeloid CFU in the marrow. Megakaryocytopoiesis is enhanced by MSC cotransplantation as assessed by an increase in both marrow CFU-MK and circulating human platelets. In contrast, MSC do not affect the percentage of human bone marrow cells that expresses CD34+. Conclusions. Cotransplantation of human mesenchymal stem cells with CD34+-selected hematopoietic stem cells enhances myelopoiesis and megakaryocytopoiesis.
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U2 - 10.1016/S0301-472X(03)00042-0
DO - 10.1016/S0301-472X(03)00042-0
M3 - Article
C2 - 12763140
AN - SCOPUS:0037562968
SN - 0301-472X
VL - 31
SP - 413
EP - 420
JO - Experimental Hematology
JF - Experimental Hematology
IS - 5
ER -