Cotranslational biogenesis of NF-κB p50 by the 26S proteasome

Li Lin, George N. DeMartino, Warner C. Greene

Research output: Contribution to journalArticle

Abstract

The NFKB1 gene encodes two functionally distinct proteins termed p50 and p105. p50 corresponds to the N terminus of p105 and with p65 (RelA) forms the prototypical NF-κB transcription factor complex. In contrast, p105 functions as a Rel-specific inhibitor (IκB) and has been proposed to be the precursor of p50. Our studies now demonstrate that p50 is generated by a unique cotranslational processing event involving the 26S proteasome, whereas cotranslational folding of sequences near the C terminus of p50 abrogates proteasome processing and leads to p105 production. These results indicate that p105 is not the precursor of p50 and reveal a novel mechanism of gene regulation that ensures the balanced production and independent function of the p50 and p105 proteins.

Original languageEnglish (US)
Pages (from-to)819-828
Number of pages10
JournalCell
Volume92
Issue number6
DOIs
StatePublished - Mar 20 1998
Externally publishedYes

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Proteasome Endopeptidase Complex
Processing
Gene expression
Genes
Proteins
Transcription Factors
ATP dependent 26S protease
3'-(1-butylphosphoryl)adenosine

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Cotranslational biogenesis of NF-κB p50 by the 26S proteasome. / Lin, Li; DeMartino, George N.; Greene, Warner C.

In: Cell, Vol. 92, No. 6, 20.03.1998, p. 819-828.

Research output: Contribution to journalArticle

Lin, L, DeMartino, GN & Greene, WC 1998, 'Cotranslational biogenesis of NF-κB p50 by the 26S proteasome', Cell, vol. 92, no. 6, pp. 819-828. https://doi.org/10.1016/S0092-8674(00)81409-9
Lin, Li ; DeMartino, George N. ; Greene, Warner C. / Cotranslational biogenesis of NF-κB p50 by the 26S proteasome. In: Cell. 1998 ; Vol. 92, No. 6. pp. 819-828.
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