Cotranslatioaal dimerization of the Rel homology domain of NF-κB1 generates p50-p105 heterodimers and is required for effective p50 production

Li Lin, George N. DeMartino, Warner C. Greene

    Research output: Contribution to journalArticle

    Abstract

    Generation of the NF-κB p50 transcription factor is mediated by the proteasome. We found previously that p50 is generated during translation of the NFKB1 gene and that this cotranslational processing allows the production of both p50 and p105 from a single mRNA. We now demonstrate that the Rel homology domain in p50 undergoes cotranslational dimerization and that this interaction is required for efficient production of p50. We further show that this coupling of dimerization and proteasome processing during translation uniquely generates p50-p105 heterodimers. Accordingly, after the primary cotranslational event, additional posttranslational steps regulate p50 homodimer formation and the intracellular ratio of p50 and p105. This cellular strategy places p50 under the control of the p105 inhibitor early in its biogenesis, thereby regulating the pool of p50 homodimers within the cell.

    Original languageEnglish (US)
    Pages (from-to)4712-4722
    Number of pages11
    JournalThe EMBO journal
    Volume19
    Issue number17
    StatePublished - Sep 1 2000

    Keywords

    • Cotranslational dimerization
    • Cotranslational processing
    • NF-κB1
    • Proteasome

    ASJC Scopus subject areas

    • Genetics
    • Cell Biology

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