Costimulatory markers in muscle of patients with idiopathic inflammatory myopathies and in cultured muscle cells

Kanneboyina Nagaraju, Nina Raben, Maria L. Villalba, Carol Danning, Lisa A. Loeffler, Eunice Lee, Nancy Tresser, Andrea Abati, Patricia Fetsch, Paul H. Plotz

Research output: Contribution to journalArticle

Abstract

In an attempt to understand the mechanisms of cell injury in the inflammatory myopathies, we analyzed the expression of costimulatory molecules, CTLA4, CD28, CD86, CD40, and CD154 as well as HLA class I, HLA class II, and ICAM-I in normal muscle and in muscle biopsies from patients with polymyositis (PM) or dermatomyositis (DM). By immunohistochemical staining, DM and PM biopsies showed the presence of CTLA4, CD28, CD86, and CD40 on inflammatory cells. More strikingly, however, low levels of CTLA4 and CD28 were observed on muscle cells. The expression of CTLA4 and CD28 on nonlymphoid cells has not been previously reported. These unexpected findings were confirmed in cultured normal human myoblasts: various proinflammatory cytokines induced the expression of CTLA4 and CD28 on normal human muscle cells. The sequences of the cDNAs were found to be identical to the sequences for these molecules in T cells. The data suggest a novel complexity in the network of cellular interactions between the infiltrated immune cells and the muscle cells in which the normal relationship between infiltrating inflammatory cells and target tissue is under a previously unrecognized set of controls.

Original languageEnglish (US)
Pages (from-to)161-169
Number of pages9
JournalClinical Immunology
Volume92
Issue number2
DOIs
StatePublished - Aug 1999
Externally publishedYes

Keywords

  • CD154
  • CD28
  • CD40
  • CD86
  • Costimulatory molecules
  • CTLA4
  • Human skeletal muscle cells
  • Myositis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

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  • Cite this

    Nagaraju, K., Raben, N., Villalba, M. L., Danning, C., Loeffler, L. A., Lee, E., Tresser, N., Abati, A., Fetsch, P., & Plotz, P. H. (1999). Costimulatory markers in muscle of patients with idiopathic inflammatory myopathies and in cultured muscle cells. Clinical Immunology, 92(2), 161-169. https://doi.org/10.1006/clim.1999.4743