TY - JOUR
T1 - Correlation of vasoactive intestinal peptide and nitric oxide synthase with choline acetyltransferase in the airway innervation
AU - Fischer, Axel
AU - Canning, Brendan J.
AU - Kummer, Wolfgang
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1996
Y1 - 1996
N2 - Vasoactive intestinal peptide (VIP) and nitric oxide (NO) are potent mediators of neural airway smooth muscle relaxation. The major contractile mediator released by airway nerves under physiological conditions is acetylcholine (ACh). In the present study, we have correlated the immunohistochemical distribution of the relaxant mediators using antisera to VIP, to the marker enzyme of catecholamine synthesis tyrosine hydroxylase (TH) and to the NO-generating enzyme NO-synthase (NOS) with the distribution of the ACh-synthesizing enzyme, choline acetyltransferase (ChAT), and of substance P (SP), a neuropeptide present in sensory nerve fibers. In guinea-pig airways, VIP- and NOS-immunoreactivity (IR) were present in numerous nerve fibers in the airway smooth muscle and around submucosal glands; some fibers were also seen in the lamina propria and around blood vessels. The neuronal cell bodies in the intrinsic ganglia were devoid of both VIP- and NOS-IR. In contrast, all neuronal cell bodies in the intrinsic ganglia were immunoreactive for ChAT. In human airways, immunoreactivity for VIP, NOS, and ChAT was found in airway intrinsic neuronal perikarya. Whereas ChAT-IR appeared to be most frequent in the cell bodies, VIP-IR was seen in the largest number of nerve fibers in the airways. Therefore, in guinea pigs, a clear neuroanatomical and neurochemical separation of relaxant and of constrictor pathways is seen, whereas in human airways, both separate pathways as well as coexpression of VIP-/NOS- and of ChAT-IR are found.
AB - Vasoactive intestinal peptide (VIP) and nitric oxide (NO) are potent mediators of neural airway smooth muscle relaxation. The major contractile mediator released by airway nerves under physiological conditions is acetylcholine (ACh). In the present study, we have correlated the immunohistochemical distribution of the relaxant mediators using antisera to VIP, to the marker enzyme of catecholamine synthesis tyrosine hydroxylase (TH) and to the NO-generating enzyme NO-synthase (NOS) with the distribution of the ACh-synthesizing enzyme, choline acetyltransferase (ChAT), and of substance P (SP), a neuropeptide present in sensory nerve fibers. In guinea-pig airways, VIP- and NOS-immunoreactivity (IR) were present in numerous nerve fibers in the airway smooth muscle and around submucosal glands; some fibers were also seen in the lamina propria and around blood vessels. The neuronal cell bodies in the intrinsic ganglia were devoid of both VIP- and NOS-IR. In contrast, all neuronal cell bodies in the intrinsic ganglia were immunoreactive for ChAT. In human airways, immunoreactivity for VIP, NOS, and ChAT was found in airway intrinsic neuronal perikarya. Whereas ChAT-IR appeared to be most frequent in the cell bodies, VIP-IR was seen in the largest number of nerve fibers in the airways. Therefore, in guinea pigs, a clear neuroanatomical and neurochemical separation of relaxant and of constrictor pathways is seen, whereas in human airways, both separate pathways as well as coexpression of VIP-/NOS- and of ChAT-IR are found.
UR - http://www.scopus.com/inward/record.url?scp=0030455524&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030455524&partnerID=8YFLogxK
U2 - 10.1111/j.1749-6632.1996.tb17547.x
DO - 10.1111/j.1749-6632.1996.tb17547.x
M3 - Article
C2 - 8993467
AN - SCOPUS:0030455524
SN - 0077-8923
VL - 805
SP - 717
EP - 722
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -