TY - JOUR
T1 - Correlation of specific keratins with different types of epithelial differentiation
T2 - Monoclonal antibody studies
AU - Tseng, Scheffer C.G.
AU - Jarvinen, Mikko J.
AU - Nelson, William G.
AU - Huang, Jing Wei
AU - Woodcock-Mitchell, Janet
AU - Sun, Tung Tien
N1 - Funding Information:
Gel Electrophoresis and lmmunoblot Technique Proteins were separated by SDS-polyacrylamide gel electrophoresis (12.5% acrylamide) as described by Laemmli (1970). The binding of (antikeratin) antibodies to keratins separated by SDS-polyacrylamide gel electrophoresis was assayed by the immunoblot technique (Tow-bin et al., 1979). Proteins from unstained polyacrylamide gels were transferred electrophoretically onto nitrocellulose paper Uvlillipore) by an E-C blotting apparatus (2.5 hr at 4°C with a power supply setting of 65%). then stained with the monoclonal antibodies by the peroxidase-antiperoxidase technique (Sternberger. 1979; Glass et al., 1980; Woodcock-Mitchell et al., 1982). Acknowledgments We thank Paula Bonitz and Helen Santana for excellent technical assistance, Riva Eichner for valuable discussions and critical reading of the manuscript, Linda Pottillo for typing, and Irwin M. Freedberg for support and encouragement. This investigation was aided in part by grants from the National Institutes of Health and by a grant from the Estee Lauder Company. T.-T. S. was a recipient of a Research Career Development Award from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
PY - 1982/9
Y1 - 1982/9
N2 - We have prepared three monoclonal antibodies against human epidermal keratins. These antibodies were highly specific for keratins and, in combination, recognized all major epidermal keratins of several mammalian species. We have used these antibodies to study the tissue distribution of epidermis-related keratins. In various mammalian epithelia, the antibodies recognized seven classes of keratins defined by their immunological reactivity and size. The 40, 46 and 52 kilodalton (kd) keratin classes were present In almost all epithelia; the 50 kd and 58 kd keratin classes were detected in all stratified squamous epithelia, but not in any simple epithelia; and the 56 kd and 65-67 kd keratin classes were unique to keratinized epidermis. Thus the expression of specific keratin classes appeared to correlate with different types of epithelial differentiation (simple versus stratified; keratinized versus nonkeratinized).
AB - We have prepared three monoclonal antibodies against human epidermal keratins. These antibodies were highly specific for keratins and, in combination, recognized all major epidermal keratins of several mammalian species. We have used these antibodies to study the tissue distribution of epidermis-related keratins. In various mammalian epithelia, the antibodies recognized seven classes of keratins defined by their immunological reactivity and size. The 40, 46 and 52 kilodalton (kd) keratin classes were present In almost all epithelia; the 50 kd and 58 kd keratin classes were detected in all stratified squamous epithelia, but not in any simple epithelia; and the 56 kd and 65-67 kd keratin classes were unique to keratinized epidermis. Thus the expression of specific keratin classes appeared to correlate with different types of epithelial differentiation (simple versus stratified; keratinized versus nonkeratinized).
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U2 - 10.1016/0092-8674(82)90234-3
DO - 10.1016/0092-8674(82)90234-3
M3 - Article
C2 - 6183000
AN - SCOPUS:0020187802
SN - 0092-8674
VL - 30
SP - 361
EP - 372
JO - Cell
JF - Cell
IS - 2
ER -