Correlation of serum measures of nitric oxide production with lupus disease activity

Gary Gilkeson, Chad Cannon, James Oates, Chris Reilly, Daniel Goldman, Michelle Petri

Research output: Contribution to journalArticle

Abstract

Objective. To determine whether serum measures of nitric oxide production correlate with disease activity in patients with systemic lupus erythematosus (SLE). Methods. We assayed the levels of serum nitrate/nitrite from 26 patients with SLE followed for 1-3 years and nitrotyrosine levels in sera from 28 additional patients with SLE; sera from 19 controls were tested in both assays. Lupus disease activity was determined via the physician's global assessment, the Lupus Activity Index, and the SLE Disease Activity Index (SLEDAI) at the time of serum collection for the initial set of 26 patients. Statistical correlations were determined using the Wilcoxon rank sum method and one-way ANOVA testing. Results. Serum levels of nitrate/nitrite were significantly higher in 26 patients with SLE compared to 19 controls (SLE, mean 29.5 μM/ml, range 1-438; controls, mean 9.6 μM/ml, range 0-51; p = 0.0004). Overall, there was a significant correlation between serum nitrate/nitrite levels and SLEDAI scores (p = 0.0065). Renal variables within the SLEDAI had the highest correlation with serum nitrate/nitrite (p = 0.0028). Serum nitrotyrosine levels were also significantly higher in patients with SLE versus controls (p = 0.007) and in active SLE versus those with inactive SLE (p = 0.008). Conclusion. Serum nitrate/nitrite levels correlated with SLE disease activity, especially nephritis, in the majority of patients studied. Serum nitrotyrosine levels also differentiated controls from patients with lupus and patients with active from those with inactive disease. Due to the ease and low cost of these assays, serum measures of nitric oxide production appear a potentially useful adjunctive laboratory measure of disease activity in SLE and further implicate nitric oxide as an important mediator of disease in SLE.

Original languageEnglish (US)
Pages (from-to)318-324
Number of pages7
JournalJournal of Rheumatology
Volume26
Issue number2
StatePublished - 1999
Externally publishedYes

Fingerprint

Systemic Lupus Erythematosus
Nitric Oxide
Serum
Nitrites
Nitrates
Nephritis
Analysis of Variance
Physicians
Kidney
Costs and Cost Analysis

Keywords

  • Disease Activity
  • Glomerulonephritis
  • Lupus
  • Nitric Oxide

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Correlation of serum measures of nitric oxide production with lupus disease activity. / Gilkeson, Gary; Cannon, Chad; Oates, James; Reilly, Chris; Goldman, Daniel; Petri, Michelle.

In: Journal of Rheumatology, Vol. 26, No. 2, 1999, p. 318-324.

Research output: Contribution to journalArticle

Gilkeson, Gary ; Cannon, Chad ; Oates, James ; Reilly, Chris ; Goldman, Daniel ; Petri, Michelle. / Correlation of serum measures of nitric oxide production with lupus disease activity. In: Journal of Rheumatology. 1999 ; Vol. 26, No. 2. pp. 318-324.
@article{703ff73bb61147b59f31954f3c25847b,
title = "Correlation of serum measures of nitric oxide production with lupus disease activity",
abstract = "Objective. To determine whether serum measures of nitric oxide production correlate with disease activity in patients with systemic lupus erythematosus (SLE). Methods. We assayed the levels of serum nitrate/nitrite from 26 patients with SLE followed for 1-3 years and nitrotyrosine levels in sera from 28 additional patients with SLE; sera from 19 controls were tested in both assays. Lupus disease activity was determined via the physician's global assessment, the Lupus Activity Index, and the SLE Disease Activity Index (SLEDAI) at the time of serum collection for the initial set of 26 patients. Statistical correlations were determined using the Wilcoxon rank sum method and one-way ANOVA testing. Results. Serum levels of nitrate/nitrite were significantly higher in 26 patients with SLE compared to 19 controls (SLE, mean 29.5 μM/ml, range 1-438; controls, mean 9.6 μM/ml, range 0-51; p = 0.0004). Overall, there was a significant correlation between serum nitrate/nitrite levels and SLEDAI scores (p = 0.0065). Renal variables within the SLEDAI had the highest correlation with serum nitrate/nitrite (p = 0.0028). Serum nitrotyrosine levels were also significantly higher in patients with SLE versus controls (p = 0.007) and in active SLE versus those with inactive SLE (p = 0.008). Conclusion. Serum nitrate/nitrite levels correlated with SLE disease activity, especially nephritis, in the majority of patients studied. Serum nitrotyrosine levels also differentiated controls from patients with lupus and patients with active from those with inactive disease. Due to the ease and low cost of these assays, serum measures of nitric oxide production appear a potentially useful adjunctive laboratory measure of disease activity in SLE and further implicate nitric oxide as an important mediator of disease in SLE.",
keywords = "Disease Activity, Glomerulonephritis, Lupus, Nitric Oxide",
author = "Gary Gilkeson and Chad Cannon and James Oates and Chris Reilly and Daniel Goldman and Michelle Petri",
year = "1999",
language = "English (US)",
volume = "26",
pages = "318--324",
journal = "Journal of Rheumatology",
issn = "0315-162X",
publisher = "Journal of Rheumatology",
number = "2",

}

TY - JOUR

T1 - Correlation of serum measures of nitric oxide production with lupus disease activity

AU - Gilkeson, Gary

AU - Cannon, Chad

AU - Oates, James

AU - Reilly, Chris

AU - Goldman, Daniel

AU - Petri, Michelle

PY - 1999

Y1 - 1999

N2 - Objective. To determine whether serum measures of nitric oxide production correlate with disease activity in patients with systemic lupus erythematosus (SLE). Methods. We assayed the levels of serum nitrate/nitrite from 26 patients with SLE followed for 1-3 years and nitrotyrosine levels in sera from 28 additional patients with SLE; sera from 19 controls were tested in both assays. Lupus disease activity was determined via the physician's global assessment, the Lupus Activity Index, and the SLE Disease Activity Index (SLEDAI) at the time of serum collection for the initial set of 26 patients. Statistical correlations were determined using the Wilcoxon rank sum method and one-way ANOVA testing. Results. Serum levels of nitrate/nitrite were significantly higher in 26 patients with SLE compared to 19 controls (SLE, mean 29.5 μM/ml, range 1-438; controls, mean 9.6 μM/ml, range 0-51; p = 0.0004). Overall, there was a significant correlation between serum nitrate/nitrite levels and SLEDAI scores (p = 0.0065). Renal variables within the SLEDAI had the highest correlation with serum nitrate/nitrite (p = 0.0028). Serum nitrotyrosine levels were also significantly higher in patients with SLE versus controls (p = 0.007) and in active SLE versus those with inactive SLE (p = 0.008). Conclusion. Serum nitrate/nitrite levels correlated with SLE disease activity, especially nephritis, in the majority of patients studied. Serum nitrotyrosine levels also differentiated controls from patients with lupus and patients with active from those with inactive disease. Due to the ease and low cost of these assays, serum measures of nitric oxide production appear a potentially useful adjunctive laboratory measure of disease activity in SLE and further implicate nitric oxide as an important mediator of disease in SLE.

AB - Objective. To determine whether serum measures of nitric oxide production correlate with disease activity in patients with systemic lupus erythematosus (SLE). Methods. We assayed the levels of serum nitrate/nitrite from 26 patients with SLE followed for 1-3 years and nitrotyrosine levels in sera from 28 additional patients with SLE; sera from 19 controls were tested in both assays. Lupus disease activity was determined via the physician's global assessment, the Lupus Activity Index, and the SLE Disease Activity Index (SLEDAI) at the time of serum collection for the initial set of 26 patients. Statistical correlations were determined using the Wilcoxon rank sum method and one-way ANOVA testing. Results. Serum levels of nitrate/nitrite were significantly higher in 26 patients with SLE compared to 19 controls (SLE, mean 29.5 μM/ml, range 1-438; controls, mean 9.6 μM/ml, range 0-51; p = 0.0004). Overall, there was a significant correlation between serum nitrate/nitrite levels and SLEDAI scores (p = 0.0065). Renal variables within the SLEDAI had the highest correlation with serum nitrate/nitrite (p = 0.0028). Serum nitrotyrosine levels were also significantly higher in patients with SLE versus controls (p = 0.007) and in active SLE versus those with inactive SLE (p = 0.008). Conclusion. Serum nitrate/nitrite levels correlated with SLE disease activity, especially nephritis, in the majority of patients studied. Serum nitrotyrosine levels also differentiated controls from patients with lupus and patients with active from those with inactive disease. Due to the ease and low cost of these assays, serum measures of nitric oxide production appear a potentially useful adjunctive laboratory measure of disease activity in SLE and further implicate nitric oxide as an important mediator of disease in SLE.

KW - Disease Activity

KW - Glomerulonephritis

KW - Lupus

KW - Nitric Oxide

UR - http://www.scopus.com/inward/record.url?scp=0032925391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032925391&partnerID=8YFLogxK

M3 - Article

VL - 26

SP - 318

EP - 324

JO - Journal of Rheumatology

JF - Journal of Rheumatology

SN - 0315-162X

IS - 2

ER -