Correlation of phospholipid hydroperoxide glutathione peroxidase activity to the sensitivity of rat retinas to photic injury

Lu Wang, Tim T. Lam, Lam W. Kwok-Wai, Mark O M Tso

Research output: Contribution to journalArticle

Abstract

We hypothesize that the differential susceptibility to photic injury among different strains of rat retinas may depend on the levels of phospholipid hydroperoxide glutathione peroxidase (PHGPX) activity, one of the endogenous antioxidant enzymes in the retina. The retinas of four inbred strains of albino rat (Fischer, Wistar, Buffalo and Lewis) were analyzed for glutathione peroxidase activity using H2O2, cumene hydroperoxide, and phospholipid hydroperoxide as assay substrates. In all four strains of rat, PHGPX was observed only in the high salt extract of the retina, while peroxidases determined by H2O2 or cumene hydroperoxide were observed mainly in the low salt extract. PHGPX was highest (66.7 mU/mg) in the most light-resistant Fischer strain and lowest in the most light- sensitive Lewis strain (31.9 mU/mg), while the activity levels in the moderately light-sensitive Buffalo and Wistar strains were 46.6 and 38.5 mU/mg, respectively. In contrast, there was no significant difference in peroxidases determined by H2O2 or cumene hydroperoxide among the four strains. These observations suggested that, in rat retina, the membrane-associated PHGPX may have an important role in the defense against light-induced free radical damage.

Original languageEnglish (US)
Pages (from-to)60-64
Number of pages5
JournalOphthalmic Research
Volume26
Issue number1
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

phospholipid-hydroperoxide glutathione peroxidase
Retina
Wounds and Injuries
Light
Peroxidases
Buffaloes
Salts
Inbred Strains Rats
Glutathione Peroxidase
Hydrogen Peroxide
Free Radicals
Phospholipids
Antioxidants
Membranes

Keywords

  • Cumene
  • Free radicals
  • Glutathione
  • Hydrogen peroxide
  • Light
  • Peroxidase
  • Phospholipid hydroperoxide
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Cellular and Molecular Neuroscience
  • Sensory Systems

Cite this

Correlation of phospholipid hydroperoxide glutathione peroxidase activity to the sensitivity of rat retinas to photic injury. / Wang, Lu; Lam, Tim T.; Kwok-Wai, Lam W.; Tso, Mark O M.

In: Ophthalmic Research, Vol. 26, No. 1, 1994, p. 60-64.

Research output: Contribution to journalArticle

@article{3992c1b028b246b7b76e6669f3e6d123,
title = "Correlation of phospholipid hydroperoxide glutathione peroxidase activity to the sensitivity of rat retinas to photic injury",
abstract = "We hypothesize that the differential susceptibility to photic injury among different strains of rat retinas may depend on the levels of phospholipid hydroperoxide glutathione peroxidase (PHGPX) activity, one of the endogenous antioxidant enzymes in the retina. The retinas of four inbred strains of albino rat (Fischer, Wistar, Buffalo and Lewis) were analyzed for glutathione peroxidase activity using H2O2, cumene hydroperoxide, and phospholipid hydroperoxide as assay substrates. In all four strains of rat, PHGPX was observed only in the high salt extract of the retina, while peroxidases determined by H2O2 or cumene hydroperoxide were observed mainly in the low salt extract. PHGPX was highest (66.7 mU/mg) in the most light-resistant Fischer strain and lowest in the most light- sensitive Lewis strain (31.9 mU/mg), while the activity levels in the moderately light-sensitive Buffalo and Wistar strains were 46.6 and 38.5 mU/mg, respectively. In contrast, there was no significant difference in peroxidases determined by H2O2 or cumene hydroperoxide among the four strains. These observations suggested that, in rat retina, the membrane-associated PHGPX may have an important role in the defense against light-induced free radical damage.",
keywords = "Cumene, Free radicals, Glutathione, Hydrogen peroxide, Light, Peroxidase, Phospholipid hydroperoxide, Retina",
author = "Lu Wang and Lam, {Tim T.} and Kwok-Wai, {Lam W.} and Tso, {Mark O M}",
year = "1994",
doi = "10.1159/000267376",
language = "English (US)",
volume = "26",
pages = "60--64",
journal = "Ophthalmic Research",
issn = "0030-3747",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Correlation of phospholipid hydroperoxide glutathione peroxidase activity to the sensitivity of rat retinas to photic injury

AU - Wang, Lu

AU - Lam, Tim T.

AU - Kwok-Wai, Lam W.

AU - Tso, Mark O M

PY - 1994

Y1 - 1994

N2 - We hypothesize that the differential susceptibility to photic injury among different strains of rat retinas may depend on the levels of phospholipid hydroperoxide glutathione peroxidase (PHGPX) activity, one of the endogenous antioxidant enzymes in the retina. The retinas of four inbred strains of albino rat (Fischer, Wistar, Buffalo and Lewis) were analyzed for glutathione peroxidase activity using H2O2, cumene hydroperoxide, and phospholipid hydroperoxide as assay substrates. In all four strains of rat, PHGPX was observed only in the high salt extract of the retina, while peroxidases determined by H2O2 or cumene hydroperoxide were observed mainly in the low salt extract. PHGPX was highest (66.7 mU/mg) in the most light-resistant Fischer strain and lowest in the most light- sensitive Lewis strain (31.9 mU/mg), while the activity levels in the moderately light-sensitive Buffalo and Wistar strains were 46.6 and 38.5 mU/mg, respectively. In contrast, there was no significant difference in peroxidases determined by H2O2 or cumene hydroperoxide among the four strains. These observations suggested that, in rat retina, the membrane-associated PHGPX may have an important role in the defense against light-induced free radical damage.

AB - We hypothesize that the differential susceptibility to photic injury among different strains of rat retinas may depend on the levels of phospholipid hydroperoxide glutathione peroxidase (PHGPX) activity, one of the endogenous antioxidant enzymes in the retina. The retinas of four inbred strains of albino rat (Fischer, Wistar, Buffalo and Lewis) were analyzed for glutathione peroxidase activity using H2O2, cumene hydroperoxide, and phospholipid hydroperoxide as assay substrates. In all four strains of rat, PHGPX was observed only in the high salt extract of the retina, while peroxidases determined by H2O2 or cumene hydroperoxide were observed mainly in the low salt extract. PHGPX was highest (66.7 mU/mg) in the most light-resistant Fischer strain and lowest in the most light- sensitive Lewis strain (31.9 mU/mg), while the activity levels in the moderately light-sensitive Buffalo and Wistar strains were 46.6 and 38.5 mU/mg, respectively. In contrast, there was no significant difference in peroxidases determined by H2O2 or cumene hydroperoxide among the four strains. These observations suggested that, in rat retina, the membrane-associated PHGPX may have an important role in the defense against light-induced free radical damage.

KW - Cumene

KW - Free radicals

KW - Glutathione

KW - Hydrogen peroxide

KW - Light

KW - Peroxidase

KW - Phospholipid hydroperoxide

KW - Retina

UR - http://www.scopus.com/inward/record.url?scp=0027982965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027982965&partnerID=8YFLogxK

U2 - 10.1159/000267376

DO - 10.1159/000267376

M3 - Article

C2 - 8134090

AN - SCOPUS:0027982965

VL - 26

SP - 60

EP - 64

JO - Ophthalmic Research

JF - Ophthalmic Research

SN - 0030-3747

IS - 1

ER -