Correlation of multidrug resistance with decreased drug accumulation, altered subcellular drug distribution, and increased P-glycoprotein expression in cultured SW-1573 human lung tumor cells

H. G. Keizer, G. J. Schuurhuis, H. J. Broxterman, J. Lankelma, W. G E J Schoonen, J. Van Rijn, H. M. Pinedo, H. Joenje

Research output: Contribution to journalArticle

Abstract

Four multidrug-resistant variants of the human squamous lung cancer cell line SW-1573 with levels of doxorubicin resistance ranging from 10- to 2000-fold were characterized with respect to drug accumulation and efflux, subcellular drug distribution pattern, antioxidant defenses, and P-glycoprotein expression. For all these parameters except the antioxidant defenses a correlation was observed with the level of doxorubicin resistance; with increasing drug resistance cellular drug accumulation capacity (as measured for doxorubicin) progressively decreased, initial drug efflux rates (as measured for daunorubicin) progressively increased, while the subcellular doxorubicin distribution (as measured by fluorescence microscopy) gradually shifted from a 'mainly nuclear' to a 'mainly cytoplasmic' pattern. Our data suggest that in the present set of cell lines the same mechanism of resistance is operating at all levels of doxorubicin resistance.

Original languageEnglish (US)
Pages (from-to)2988-2993
Number of pages6
JournalCancer Research
Volume49
Issue number11
StatePublished - 1989
Externally publishedYes

Fingerprint

Multiple Drug Resistance
P-Glycoprotein
Doxorubicin
Lung
Pharmaceutical Preparations
Neoplasms
Antioxidants
Cell Line
Daunorubicin
Fluorescence Microscopy
Drug Resistance
Lung Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Keizer, H. G., Schuurhuis, G. J., Broxterman, H. J., Lankelma, J., Schoonen, W. G. E. J., Van Rijn, J., ... Joenje, H. (1989). Correlation of multidrug resistance with decreased drug accumulation, altered subcellular drug distribution, and increased P-glycoprotein expression in cultured SW-1573 human lung tumor cells. Cancer Research, 49(11), 2988-2993.

Correlation of multidrug resistance with decreased drug accumulation, altered subcellular drug distribution, and increased P-glycoprotein expression in cultured SW-1573 human lung tumor cells. / Keizer, H. G.; Schuurhuis, G. J.; Broxterman, H. J.; Lankelma, J.; Schoonen, W. G E J; Van Rijn, J.; Pinedo, H. M.; Joenje, H.

In: Cancer Research, Vol. 49, No. 11, 1989, p. 2988-2993.

Research output: Contribution to journalArticle

Keizer, HG, Schuurhuis, GJ, Broxterman, HJ, Lankelma, J, Schoonen, WGEJ, Van Rijn, J, Pinedo, HM & Joenje, H 1989, 'Correlation of multidrug resistance with decreased drug accumulation, altered subcellular drug distribution, and increased P-glycoprotein expression in cultured SW-1573 human lung tumor cells', Cancer Research, vol. 49, no. 11, pp. 2988-2993.
Keizer, H. G. ; Schuurhuis, G. J. ; Broxterman, H. J. ; Lankelma, J. ; Schoonen, W. G E J ; Van Rijn, J. ; Pinedo, H. M. ; Joenje, H. / Correlation of multidrug resistance with decreased drug accumulation, altered subcellular drug distribution, and increased P-glycoprotein expression in cultured SW-1573 human lung tumor cells. In: Cancer Research. 1989 ; Vol. 49, No. 11. pp. 2988-2993.
@article{b34c675432934ef9ba215bccff6d5031,
title = "Correlation of multidrug resistance with decreased drug accumulation, altered subcellular drug distribution, and increased P-glycoprotein expression in cultured SW-1573 human lung tumor cells",
abstract = "Four multidrug-resistant variants of the human squamous lung cancer cell line SW-1573 with levels of doxorubicin resistance ranging from 10- to 2000-fold were characterized with respect to drug accumulation and efflux, subcellular drug distribution pattern, antioxidant defenses, and P-glycoprotein expression. For all these parameters except the antioxidant defenses a correlation was observed with the level of doxorubicin resistance; with increasing drug resistance cellular drug accumulation capacity (as measured for doxorubicin) progressively decreased, initial drug efflux rates (as measured for daunorubicin) progressively increased, while the subcellular doxorubicin distribution (as measured by fluorescence microscopy) gradually shifted from a 'mainly nuclear' to a 'mainly cytoplasmic' pattern. Our data suggest that in the present set of cell lines the same mechanism of resistance is operating at all levels of doxorubicin resistance.",
author = "Keizer, {H. G.} and Schuurhuis, {G. J.} and Broxterman, {H. J.} and J. Lankelma and Schoonen, {W. G E J} and {Van Rijn}, J. and Pinedo, {H. M.} and H. Joenje",
year = "1989",
language = "English (US)",
volume = "49",
pages = "2988--2993",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "11",

}

TY - JOUR

T1 - Correlation of multidrug resistance with decreased drug accumulation, altered subcellular drug distribution, and increased P-glycoprotein expression in cultured SW-1573 human lung tumor cells

AU - Keizer, H. G.

AU - Schuurhuis, G. J.

AU - Broxterman, H. J.

AU - Lankelma, J.

AU - Schoonen, W. G E J

AU - Van Rijn, J.

AU - Pinedo, H. M.

AU - Joenje, H.

PY - 1989

Y1 - 1989

N2 - Four multidrug-resistant variants of the human squamous lung cancer cell line SW-1573 with levels of doxorubicin resistance ranging from 10- to 2000-fold were characterized with respect to drug accumulation and efflux, subcellular drug distribution pattern, antioxidant defenses, and P-glycoprotein expression. For all these parameters except the antioxidant defenses a correlation was observed with the level of doxorubicin resistance; with increasing drug resistance cellular drug accumulation capacity (as measured for doxorubicin) progressively decreased, initial drug efflux rates (as measured for daunorubicin) progressively increased, while the subcellular doxorubicin distribution (as measured by fluorescence microscopy) gradually shifted from a 'mainly nuclear' to a 'mainly cytoplasmic' pattern. Our data suggest that in the present set of cell lines the same mechanism of resistance is operating at all levels of doxorubicin resistance.

AB - Four multidrug-resistant variants of the human squamous lung cancer cell line SW-1573 with levels of doxorubicin resistance ranging from 10- to 2000-fold were characterized with respect to drug accumulation and efflux, subcellular drug distribution pattern, antioxidant defenses, and P-glycoprotein expression. For all these parameters except the antioxidant defenses a correlation was observed with the level of doxorubicin resistance; with increasing drug resistance cellular drug accumulation capacity (as measured for doxorubicin) progressively decreased, initial drug efflux rates (as measured for daunorubicin) progressively increased, while the subcellular doxorubicin distribution (as measured by fluorescence microscopy) gradually shifted from a 'mainly nuclear' to a 'mainly cytoplasmic' pattern. Our data suggest that in the present set of cell lines the same mechanism of resistance is operating at all levels of doxorubicin resistance.

UR - http://www.scopus.com/inward/record.url?scp=0024313346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024313346&partnerID=8YFLogxK

M3 - Article

VL - 49

SP - 2988

EP - 2993

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 11

ER -