Correlating 3D morphology with molecular pathology: Fibrotic remodelling in human lung biopsies

Manuela Kellner, Judith Wehling, Gregor Warnecke, Marko Heidrich, Nicole Izykowski, Jens Vogel-Claussen, Raoul Amadeus Lorbeer, Georgios Antonopoulos, Sabina Janciauskiene, Roman Grothausmann, Lars Knudsen, Tammo Ripken, Heiko Meyer, Hans Kreipe, Matthias Ochs, Danny Jonigk, Mark Philipp Kühnel

Research output: Contribution to journalArticle

Abstract

Assessing alterations of the parenchymal architecture is essential in understanding fibrosing interstitial lung diseases. Here, we present a novel method to visualise fibrotic remodelling in human lungs and correlate morphological three-dimensional (3D) data with gene and protein expression in the very same sample. The key to our approach is a novel embedding resin that clears samples to full optical transparency and simultaneously allows 3D laser tomography and preparation of sections for histology, immunohistochemistry and RNA isolation. Correlating 3D laser tomography with molecular diagnostic techniques enables new insights into lung diseases. This approach has great potential to become an essential tool in pulmonary research.

Original languageEnglish (US)
Pages (from-to)1197-1198
Number of pages2
JournalThorax
Volume70
Issue number12
DOIs
Publication statusPublished - Dec 1 2015
Externally publishedYes

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ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Kellner, M., Wehling, J., Warnecke, G., Heidrich, M., Izykowski, N., Vogel-Claussen, J., ... Kühnel, M. P. (2015). Correlating 3D morphology with molecular pathology: Fibrotic remodelling in human lung biopsies. Thorax, 70(12), 1197-1198. https://doi.org/10.1136/thoraxjnl-2015-207131