Correcting reaction rates measured by saturation-transfer magnetic resonance spectroscopy

Research output: Contribution to journalArticle

Abstract

Off-resonance or spillover irradiation and incomplete saturation can introduce significant errors in the estimates of chemical rate constants measured by saturation-transfer magnetic resonance spectroscopy (MRS). Existing methods of correction are effective only over a limited parameter range. Here, a general approach of numerically solving the Bloch-McConnell equations to calculate exchange rates, relaxation times and concentrations for the saturation-transfer experiment is investigated, but found to require more measurements and higher signal-to-noise ratios than in vivo studies can practically afford. As an alternative, correction formulae for the reaction rate are provided which account for the expected parameter ranges and limited measurements available in vivo. The correction term is a quadratic function of experimental measurements. In computer simulations, the new formulae showed negligible bias and reduced the maximum error in the rate constants by about 3-fold compared to traditional formulae, and the error scatter by about 4-fold, over a wide range of parameters for conventional saturation transfer employing progressive saturation, and for the four-angle saturation-transfer method applied to the creatine kinase (CK) reaction in the human heart at 1.5 T. In normal in vivo spectra affected by spillover, the correction increases the mean calculated forward CK reaction rate by 6-16% over traditional and prior correction formulae.

Original languageEnglish (US)
Pages (from-to)248-258
Number of pages11
JournalJournal of Magnetic Resonance
Volume191
Issue number2
DOIs
StatePublished - Apr 1 2008

Keywords

  • Chemical exchange
  • Creatine kinase
  • Forward reaction rate
  • Magnetic resonance spectroscopy
  • Saturation transfer

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

Fingerprint Dive into the research topics of 'Correcting reaction rates measured by saturation-transfer magnetic resonance spectroscopy'. Together they form a unique fingerprint.

  • Cite this