Corneal damage from exposure to IR radiation: Rabbit endothelial damage thresholds

C. Brent Bargeron, Richard A. Farrell, William R. Green, Russell L. Mc Cally

Research output: Contribution to journalArticle

Abstract

Rabbit endothelial damage thresholds have been determined for exposure to CO2 laser radiation (10.6 μm). The laser was operated in the TEM00 mode (Gaussian irradiance profile), and the 1/e diameter of the beam was 2.0 mm. Endothelial damage was detected using a staining technique. Threshold damage is characterized by distorted cells and uneven staining of the cell borders; while at exposure times of up to 90% of the threshold, the stained endothelia were indistinguishable from those in control eyes. Three fixed peak irradiance levels (24.5, 10.0 and 3.6 W/cm2) were investigated and the exposure duration was varied until threshold damage occurred. In each instance the threshold exposure duration for endothelial damage (1.0, 5.2 and 240 sec, respectively) was about 10 or more times the accepted threshold duration for epithelial damage. These results suggest that protecting against epithelial damage will also ensure against endothelial damage for CO2 laser systems. However, the calculated peak endothelial temperature increases at these threshold exposures are similar to those of the epithelium at its damage threshold. Thus, as discussed in the text, other IR laser systems could harm the endothelium at epithelial damage threshold exposure levels.

Original languageEnglish (US)
Pages (from-to)855-862
Number of pages8
JournalHealth Physics
Volume40
Issue number6
StatePublished - 1981

Fingerprint

Gas Lasers
rabbits
yield point
Endothelium
Lasers
Radiation
Staining and Labeling
Rabbits
damage
Infrared radiation
radiation
endothelium
thresholds
Epithelium
staining
Laser radiation
Laser modes
irradiance
laser
Temperature

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Epidemiology
  • Radiology Nuclear Medicine and imaging
  • Environmental Science(all)
  • Environmental Science (miscellaneous)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Toxicology
  • Physics and Astronomy (miscellaneous)

Cite this

Bargeron, C. B., Farrell, R. A., Green, W. R., & Mc Cally, R. L. (1981). Corneal damage from exposure to IR radiation: Rabbit endothelial damage thresholds. Health Physics, 40(6), 855-862.

Corneal damage from exposure to IR radiation : Rabbit endothelial damage thresholds. / Bargeron, C. Brent; Farrell, Richard A.; Green, William R.; Mc Cally, Russell L.

In: Health Physics, Vol. 40, No. 6, 1981, p. 855-862.

Research output: Contribution to journalArticle

Bargeron, CB, Farrell, RA, Green, WR & Mc Cally, RL 1981, 'Corneal damage from exposure to IR radiation: Rabbit endothelial damage thresholds', Health Physics, vol. 40, no. 6, pp. 855-862.
Bargeron CB, Farrell RA, Green WR, Mc Cally RL. Corneal damage from exposure to IR radiation: Rabbit endothelial damage thresholds. Health Physics. 1981;40(6):855-862.
Bargeron, C. Brent ; Farrell, Richard A. ; Green, William R. ; Mc Cally, Russell L. / Corneal damage from exposure to IR radiation : Rabbit endothelial damage thresholds. In: Health Physics. 1981 ; Vol. 40, No. 6. pp. 855-862.
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