Coreceptor utilization of HIV type 1 subtype E viral isolates from Thai men with HIV type 1-infected and Uninfected Wives

Utaiwan Utaipat, Ann Duerr, Donna L. Rudolph, Chunfu Yang, Salvatore T. Butera, Davis Lupo, Tracy Pisell, Arunrat Tangmunkongvorakul, Nuonchuen Kamtorn, Niwes Nantachit, Tippavan Nagachinta, Vinai Suriyanon, Valerie Robison, Kenrad E. Nelson, Nopporn Sittisombut, Renu B. Lal

Research output: Contribution to journalArticle

Abstract

HIV-1 coreceptors CCR5 and CXCR4 play an important role in viral entry and pathogenesis. To better understand the role of viral tropism in HIV-1 transmission, we examined the coreceptor utilization of viral isolates obtained from men enrolled in a study of heterosexual transmission in northern Thailand. Viral isolates were obtained from HIV-1-positive males who had either HIV-1-infected spouses (RM; n = 5) or HIV-1-uninfected spouses (HM; n = 10). Viral isolates from 1 of the 5 RM males and 2 of the 10 HM males were CCR5 tropic, whereas isolates from 3 RM males and 6 of the HM male isolates were CXCR4 tropic. Of the nine X4-tropic isolates, seven also used at least one of the following coreceptors: CCR8, CCR1, CCR2b, or CX3CR1, and none employed CCR5 as an additional coreceptor. More importantly, three isolates, RM-15, HM-13, and HM-16 (one from a transmitter and two from nontransmitter), did not infect GHOST4.cl.34 cells expressing any of the known coreceptors. Further analysis using MAGI-plaque assays, which allow visualization of infected cells, revealed that RM-15 had low numbers of infected cells in MAGI-R5 and MAGI-X4 cultures, whereas HM-13 and HM-16 had high levels of plaques in MAGI-X4 cultures. Replication kinetics using activated lymphocytes revealed that these three isolates replicated in CCR5+/+as well as CCR5-/-peripheral blood mononuclear cells, suggesting that these isolates did not have an absolute requirement of CCR5 for viral entry. All three isolates were sensitive to the X4-antagonistic compounds T-22 and AMD3100. Analysis of the C2V3 region did not reveal any significant structural differences between any of the Thai subtype E isolates. Thus, there was no association between the pattern of coreceptor usage and transmissibility among these subtype E HIV-1 isolates.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalAIDS research and human retroviruses
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2002

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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    Utaipat, U., Duerr, A., Rudolph, D. L., Yang, C., Butera, S. T., Lupo, D., Pisell, T., Tangmunkongvorakul, A., Kamtorn, N., Nantachit, N., Nagachinta, T., Suriyanon, V., Robison, V., Nelson, K. E., Sittisombut, N., & Lal, R. B. (2002). Coreceptor utilization of HIV type 1 subtype E viral isolates from Thai men with HIV type 1-infected and Uninfected Wives. AIDS research and human retroviruses, 18(1), 1-11. https://doi.org/10.1089/088922202753394664