Copper(II)-lincomycin: Complexation pattern and oxidative activity

Małgorzata Jezowska-Bojczuk, Wojciech Lesniak, Wojciech Szczepanik, Kazimierz Gatner, Adam Jezierski, Marek Smoluch, Wojciech Bal

Research output: Contribution to journalArticle

Abstract

Coordination of Cu(II) to lincomycin was studied by potentiometry, UV-Vis, circular dichroism (CD), EPR, NMR, cyclic voltammetry (CV) and ESI-MS. Only mononuclear complexes of stoichiometries ranging from CuL to CuH-3L were found. In the main species present at neutral pH, CuH-2L, lincomycin bonds Cu(II) through both of its nitrogen donors, and a deprotonated oxygen donor at C4 of the sugar moiety. High pressure liquid chromatography (HPLC) of products of 2′-deoxy guano sine (dG) oxidation and agarose gel electrophoresis of plasmid DNA confirmed that lincomycin complexes effectively facilitate dG oxidation by H2O2, but are not able to cleave double-stranded plasmid DNA.

Original languageEnglish (US)
Pages (from-to)189-200
Number of pages12
JournalJournal of Inorganic Biochemistry
Volume84
Issue number3-4
StatePublished - 2001
Externally publishedYes

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Keywords

  • 2′-Deoxyguanosine oxidation
  • Copper(II) complexes
  • Cu(II)/Cu(III) redox pair
  • Lincomycin
  • pBR322 plasmid cleavage
  • Potentiometry
  • Spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Jezowska-Bojczuk, M., Lesniak, W., Szczepanik, W., Gatner, K., Jezierski, A., Smoluch, M., & Bal, W. (2001). Copper(II)-lincomycin: Complexation pattern and oxidative activity. Journal of Inorganic Biochemistry, 84(3-4), 189-200.