TY - JOUR
T1 - Copper(II)-lincomycin
T2 - Complexation pattern and oxidative activity
AU - Jezowska-Bojczuk, Małgorzata
AU - Leśniak, Wojciech
AU - Szczepanik, Wojciech
AU - Gatner, Kazimierz
AU - Jezierski, Adam
AU - Smoluch, Marek
AU - Bal, Wojciech
N1 - Funding Information:
The authors would like to express their gratitude to Dr. K.S. Kasprzak (NCI-FCRDC) for his kind gift of 8-oxo-dG HPLC standard. The work was supported by the Polish State Committee for Scientific Research (KBN), grant No 3 T09A 068 18.
PY - 2001
Y1 - 2001
N2 - Coordination of Cu(II) to lincomycin was studied by potentiometry, UV-Vis, circular dichroism (CD), EPR, NMR, cyclic voltammetry (CV) and ESI-MS. Only mononuclear complexes of stoichiometries ranging from CuL to CuH-3L were found. In the main species present at neutral pH, CuH-2L, lincomycin bonds Cu(II) through both of its nitrogen donors, and a deprotonated oxygen donor at C4 of the sugar moiety. High pressure liquid chromatography (HPLC) of products of 2′-deoxy guano sine (dG) oxidation and agarose gel electrophoresis of plasmid DNA confirmed that lincomycin complexes effectively facilitate dG oxidation by H2O2, but are not able to cleave double-stranded plasmid DNA.
AB - Coordination of Cu(II) to lincomycin was studied by potentiometry, UV-Vis, circular dichroism (CD), EPR, NMR, cyclic voltammetry (CV) and ESI-MS. Only mononuclear complexes of stoichiometries ranging from CuL to CuH-3L were found. In the main species present at neutral pH, CuH-2L, lincomycin bonds Cu(II) through both of its nitrogen donors, and a deprotonated oxygen donor at C4 of the sugar moiety. High pressure liquid chromatography (HPLC) of products of 2′-deoxy guano sine (dG) oxidation and agarose gel electrophoresis of plasmid DNA confirmed that lincomycin complexes effectively facilitate dG oxidation by H2O2, but are not able to cleave double-stranded plasmid DNA.
KW - 2′-Deoxyguanosine oxidation
KW - Copper(II) complexes
KW - Cu(II)/Cu(III) redox pair
KW - Lincomycin
KW - Potentiometry
KW - Spectroscopy
KW - pBR322 plasmid cleavage
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U2 - 10.1016/S0162-0134(01)00167-2
DO - 10.1016/S0162-0134(01)00167-2
M3 - Article
C2 - 11374581
AN - SCOPUS:0035317337
SN - 0162-0134
VL - 84
SP - 189
EP - 200
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
IS - 3-4
ER -