Coordination of mitochondrial bioenergetics with G1 phase cell cycle progression

Stefan M. Schieke, J. Philip McCoy, Toren Finkel

Research output: Contribution to journalArticlepeer-review

Abstract

Relatively little is known regarding how energetic demand during cell proliferation is sensed or coordinated with mitochondrial metabolism. Here we demonstrate that cell cycle progression through G1 is associated with a significant increase in mitochondrial membrane potential (Δψ m) and respiration. We used this change in metabolic rate to isolate cells in G1 with low and high levels of mitochondrial membrane potential (ΔψmL and ΔψmH). Biochemical and functional studies demonstrate that ΔψmL and ΔψmH cells display the distinct characteristics of early and late G1 phase, respectively. We further demonstrate that the metabolic rate in G1 reflects levels of the mTOR-raptor complex as well as susceptibility to rapamycin-induced cell cycle delay. In conclusion, our data suggests a coupling of mitochondrial bioenergetics and G1 progression and points to the mTOR signaling pathway as a potential molecular coordinator of these two processes.

Original languageEnglish (US)
Pages (from-to)1782-1787
Number of pages6
JournalCell cycle (Georgetown, Tex.)
Volume7
Issue number12
StatePublished - Jun 15 2008
Externally publishedYes

Keywords

  • Cell cycle
  • Mitochondria
  • mTOR

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Coordination of mitochondrial bioenergetics with G<sub>1</sub> phase cell cycle progression'. Together they form a unique fingerprint.

Cite this