Coordinated regulation of scaffold opening and enzymatic activity during CARD11 signaling

Zhaoquan Wang, Shelby M. Hutcherson, Chao Yang, Rakhi P. Jattani, Julia M. Tritapoe, Yongkang Yang, Joel L Pomerantz

Research output: Contribution to journalArticle

Abstract

The activation of key signaling pathways downstream of antigen receptor engagement is critically required for normal lymphocyte activation during the adaptive immune response. CARD11 is a multidomain signaling scaffold protein required for antigen receptor signaling to NF-κB, c-Jun N-terminal kinase, and mTOR. Germline mutations in the CARD11 gene result in at least four types of primary immunodeficiency, and somatic CARD11 gain-of-function mutations drive constitutive NF-κB activity in diffuse large B cell lymphoma and other lymphoid cancers. In response to antigen receptor triggering, CARD11 transitions from a closed, inactive state to an open, active scaffold that recruits multiple signaling partners into a complex to relay downstream signaling. However, how this signal- induced CARD11 conversion occurs remains poorly understood. Here we investigate the role of Inducible Element 1 (IE1), a short regulatory element in the CARD11 Inhibitory Domain, in the CARD11 signaling cycle. We find that IE1 controls the signal-dependent Opening Step that makes CARD11 accessible to the binding of cofactors, including Bcl10, MALT1, and the HOIP catalytic subunit of the linear ubiquitin chain assembly complex. Surprisingly, we find that IE1 is also required at an independent step for the maximal activation of HOIP and MALT1 enzymatic activity after cofactor recruitment to CARD11. This role of IE1 reveals that there is an Enzymatic Activation Step in the CARD11 signaling cycle that is distinct from the Cofactor Association Step. Our results indicate that CARD11 has evolved to actively coordinate scaffold opening and the induction of enzymatic activity among recruited cofactors during antigen receptor signaling.

Original languageEnglish (US)
Pages (from-to)14648-14660
Number of pages13
JournalJournal of Biological Chemistry
Volume294
Issue number40
DOIs
StatePublished - Jan 1 2019

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Antigen Receptors
Scaffolds
Chemical activation
Lymphoma, Large B-Cell, Diffuse
Germ-Line Mutation
JNK Mitogen-Activated Protein Kinases
Sexual Partners
Adaptive Immunity
Lymphocyte Activation
Ubiquitin
Catalytic Domain
Mutation
Lymphocytes
Genes
Neoplasms
Proteins
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Coordinated regulation of scaffold opening and enzymatic activity during CARD11 signaling. / Wang, Zhaoquan; Hutcherson, Shelby M.; Yang, Chao; Jattani, Rakhi P.; Tritapoe, Julia M.; Yang, Yongkang; Pomerantz, Joel L.

In: Journal of Biological Chemistry, Vol. 294, No. 40, 01.01.2019, p. 14648-14660.

Research output: Contribution to journalArticle

Wang, Zhaoquan ; Hutcherson, Shelby M. ; Yang, Chao ; Jattani, Rakhi P. ; Tritapoe, Julia M. ; Yang, Yongkang ; Pomerantz, Joel L. / Coordinated regulation of scaffold opening and enzymatic activity during CARD11 signaling. In: Journal of Biological Chemistry. 2019 ; Vol. 294, No. 40. pp. 14648-14660.
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