Coomassie blue staining

Julie L. Brunelle, Rachel Green

Research output: Chapter in Book/Report/Conference proceedingChapter


This protocol describes Coomassie brilliant blue staining, one of the most common methods of detecting proteins in polyacrylamide gels (PAGE).

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part C
PublisherAcademic Press Inc.
Number of pages7
ISBN (Print)9780124201194
StatePublished - 2014

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Coomassie blue staining
  • G-250
  • Kimwipes or paper towels
  • Polyacrylamide gels (PAGE)
  • R-250
  • Silver staining
  • Whatman 1 filter paper

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Brunelle, J. L., & Green, R. (2014). Coomassie blue staining. In Laboratory Methods in Enzymology: Protein Part C (pp. 161-167). (Methods in Enzymology; Vol. 541). Academic Press Inc..