Coomassie blue staining

Julie L. Brunelle, Rachel Green

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This protocol describes Coomassie brilliant blue staining, one of the most common methods of detecting proteins in polyacrylamide gels (PAGE).

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages161-167
Number of pages7
Volume541
ISBN (Print)9780124201194
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume541
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Staining and Labeling
Proteins
Coomassie blue
coomassie Brilliant Blue
polyacrylamide gels

Keywords

  • Coomassie blue staining
  • G-250
  • Kimwipes or paper towels
  • Polyacrylamide gels (PAGE)
  • R-250
  • Silver staining
  • Whatman 1 filter paper

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Brunelle, J. L., & Green, R. (2014). Coomassie blue staining. In Methods in Enzymology (Vol. 541, pp. 161-167). (Methods in Enzymology; Vol. 541). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420119-4.00013-6

Coomassie blue staining. / Brunelle, Julie L.; Green, Rachel.

Methods in Enzymology. Vol. 541 Academic Press Inc., 2014. p. 161-167 (Methods in Enzymology; Vol. 541).

Research output: Chapter in Book/Report/Conference proceedingChapter

Brunelle, JL & Green, R 2014, Coomassie blue staining. in Methods in Enzymology. vol. 541, Methods in Enzymology, vol. 541, Academic Press Inc., pp. 161-167. https://doi.org/10.1016/B978-0-12-420119-4.00013-6
Brunelle JL, Green R. Coomassie blue staining. In Methods in Enzymology. Vol. 541. Academic Press Inc. 2014. p. 161-167. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-420119-4.00013-6
Brunelle, Julie L. ; Green, Rachel. / Coomassie blue staining. Methods in Enzymology. Vol. 541 Academic Press Inc., 2014. pp. 161-167 (Methods in Enzymology).
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