TY - JOUR
T1 - Cooling evokes redistribution of α2C-adrenoceptors from Golgi to plasma membrane in transfected human embryonic kidney 293 cells
AU - Jeyaraj, S. C.
AU - Chotani, M. A.
AU - Mitra, S.
AU - Gregg, H. E.
AU - Flavahan, N. A.
AU - Morrison, K. J.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - Cold-induced vasoconstriction in cutaneous blood vessels is mediated by increased constrictor activity of vascular α2-adrenoceptors (α2-ARs). In mouse cutaneous arteries, α2-AR constriction at 37°C is mediated by α2A-ARs, whereas after cold exposure (28°C), α2C-ARs are no longer silent and mediate the remarkable cold-induced augmentation of α2-AR responsiveness. The goals of the present study were to develop a cell model of cutaneous thermoregulation and to determine the mechanisms underlying the thermosensitivity of α2C-ARs. Human embryonic kidney 293 cells were transiently transfected with the mouse α2A- or α2C-AR. In cells expressing α2A-ARs, UK-14,304 (5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine), an α2-AR agonist, inhibited (10 pM) and stimulated (1-10 nM) the accumulation of cAMP evoked by forskolin. Similar responses were obtained at 37°C and 28°C. In contrast, in cells expressing α2C-ARs, UK-14,304 did not affect forskolin-stimulated cAMP accumulation at 37°C but did cause a concentration-dependent inhibitory effect at 28°C. Subcellular fractionation revealed that at 37°C α2C-ARs were localized predominantly to Golgi compartments, whereas α2A-ARs localized predominantly to the plasma membrane. After cooling (28°C), α2C-ARs relocated from Golgi compartments to the plasma membrane, whereas the α2A-AR remained at the plasma membrane. Immunofluorescence microscopy confirmed that, at 37°C, α2A-ARs were localized to the cell surface, whereas α2C-ARs colocalized with a trans-Golgi marker. Cooling did not affect localization of α2A-ARs, but shifted α2C-ARs to the cell surface. Moderate cooling, therefore, caused a selective redistribution of α2C-ARs from the Golgi compartments to the cell surface, allowing the rescue of the α2C-adrenergic functional response. This mechanism may explain the role of α2-ARs in thermoregulation of the cutaneous circulation.
AB - Cold-induced vasoconstriction in cutaneous blood vessels is mediated by increased constrictor activity of vascular α2-adrenoceptors (α2-ARs). In mouse cutaneous arteries, α2-AR constriction at 37°C is mediated by α2A-ARs, whereas after cold exposure (28°C), α2C-ARs are no longer silent and mediate the remarkable cold-induced augmentation of α2-AR responsiveness. The goals of the present study were to develop a cell model of cutaneous thermoregulation and to determine the mechanisms underlying the thermosensitivity of α2C-ARs. Human embryonic kidney 293 cells were transiently transfected with the mouse α2A- or α2C-AR. In cells expressing α2A-ARs, UK-14,304 (5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine), an α2-AR agonist, inhibited (10 pM) and stimulated (1-10 nM) the accumulation of cAMP evoked by forskolin. Similar responses were obtained at 37°C and 28°C. In contrast, in cells expressing α2C-ARs, UK-14,304 did not affect forskolin-stimulated cAMP accumulation at 37°C but did cause a concentration-dependent inhibitory effect at 28°C. Subcellular fractionation revealed that at 37°C α2C-ARs were localized predominantly to Golgi compartments, whereas α2A-ARs localized predominantly to the plasma membrane. After cooling (28°C), α2C-ARs relocated from Golgi compartments to the plasma membrane, whereas the α2A-AR remained at the plasma membrane. Immunofluorescence microscopy confirmed that, at 37°C, α2A-ARs were localized to the cell surface, whereas α2C-ARs colocalized with a trans-Golgi marker. Cooling did not affect localization of α2A-ARs, but shifted α2C-ARs to the cell surface. Moderate cooling, therefore, caused a selective redistribution of α2C-ARs from the Golgi compartments to the cell surface, allowing the rescue of the α2C-adrenergic functional response. This mechanism may explain the role of α2-ARs in thermoregulation of the cutaneous circulation.
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U2 - 10.1124/mol.60.6.1195
DO - 10.1124/mol.60.6.1195
M3 - Article
C2 - 11723226
AN - SCOPUS:0035206445
SN - 0026-895X
VL - 60
SP - 1195
EP - 1200
JO - Molecular Pharmacology
JF - Molecular Pharmacology
IS - 6
ER -