Controlling P element insertional mutagenesis

Lynn Cooley, Celeste Berg, Allan Spradling

Research output: Contribution to journalArticle

Abstract

Two new methods for insertional mutagenesis in Drosophila are now available. They are based on the ability to control experimentally the number of stable P element insertions per mutagenized strain. One method produces about 10 insertions per genome and has the potential to replace hybrid dysgenesis as a means for cloning genes by transposon tagging. The other mobilizes elements less frequently, producing strains containing a single P element that can be correlated with any resulting mutant phenotype. A library of single-element strains is currently being assembled and will be a valuable resource for Drosophila molecular genetics.

Original languageEnglish (US)
Pages (from-to)254-258
Number of pages5
JournalTrends in Genetics
Volume4
Issue number9
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Insertional Mutagenesis
Drosophila
Libraries
Organism Cloning
Molecular Biology
Genome
Phenotype
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Controlling P element insertional mutagenesis. / Cooley, Lynn; Berg, Celeste; Spradling, Allan.

In: Trends in Genetics, Vol. 4, No. 9, 1988, p. 254-258.

Research output: Contribution to journalArticle

Cooley, L, Berg, C & Spradling, A 1988, 'Controlling P element insertional mutagenesis', Trends in Genetics, vol. 4, no. 9, pp. 254-258. https://doi.org/10.1016/0168-9525(88)90032-7
Cooley, Lynn ; Berg, Celeste ; Spradling, Allan. / Controlling P element insertional mutagenesis. In: Trends in Genetics. 1988 ; Vol. 4, No. 9. pp. 254-258.
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