TY - JOUR
T1 - Controlled-release of IGF-I and TGF-β1 in a photopolymerizing hydrogel for cartilage tissue engineering
AU - Elisseeff, Jennifer
AU - McIntosh, Winnette
AU - Fu, Karen
AU - Blunk, Torsten
AU - Langer, Robert
N1 - Funding Information:
The authors gratefully acknowledge NIH grant DE13023 for financial support. The authors also acknowledge helpful discussions with Dr. Kristi Anseth, Mark Randolph and Dr. Michael Yaremchuk.
PY - 2001
Y1 - 2001
N2 - Photopolymerizing hydrogel systems provide a method to encapsulate cells and implant materials in a minimally invasive manner. Controlled release of growth factors in the hydrogels may enhance the ability to engineer tissues. IGF-I and TGF-β were loaded in PLGA microspheres using a double emulsion technique. 125 ng and 200 pg of active IGF-I and TGF-β, respectively, as measured by ELISA, were released over 15 days. The growth factor containing microspheres were photoencapsulated with bovine articular chondrocytes in PEO-based hydrogels and incubated in vitro for two weeks. Statistically significant changes in glycosaminoglycan (GAG) production compared to control gels either without microspheres or with blank spheres were observed after a 14 day incubation with IGF-I and IGF-I/TGF-β microspheres combined, with a maximum density of 8.41 ± 2.5% wet weight GAG. Total collagen density was low and decreased with the IGF-I/TGF-β microspheres after two weeks incubation, but otherwise remained unchanged in all other experimental groups. Cell content increased 10-fold to 0.18 ± 0.056 × 106 cells/mg wet weight and extracellular matrix (ECM) staining by H&E increased in hydrogels with IGF-I/TGF-β microspheres. In conclusion, photoencapsulation of microspheres in PEO-based hydrogels provides a method to deliver molecules such as growth factors in porous hydrogel systems.
AB - Photopolymerizing hydrogel systems provide a method to encapsulate cells and implant materials in a minimally invasive manner. Controlled release of growth factors in the hydrogels may enhance the ability to engineer tissues. IGF-I and TGF-β were loaded in PLGA microspheres using a double emulsion technique. 125 ng and 200 pg of active IGF-I and TGF-β, respectively, as measured by ELISA, were released over 15 days. The growth factor containing microspheres were photoencapsulated with bovine articular chondrocytes in PEO-based hydrogels and incubated in vitro for two weeks. Statistically significant changes in glycosaminoglycan (GAG) production compared to control gels either without microspheres or with blank spheres were observed after a 14 day incubation with IGF-I and IGF-I/TGF-β microspheres combined, with a maximum density of 8.41 ± 2.5% wet weight GAG. Total collagen density was low and decreased with the IGF-I/TGF-β microspheres after two weeks incubation, but otherwise remained unchanged in all other experimental groups. Cell content increased 10-fold to 0.18 ± 0.056 × 106 cells/mg wet weight and extracellular matrix (ECM) staining by H&E increased in hydrogels with IGF-I/TGF-β microspheres. In conclusion, photoencapsulation of microspheres in PEO-based hydrogels provides a method to deliver molecules such as growth factors in porous hydrogel systems.
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U2 - 10.1016/S0736-0266(01)00054-7
DO - 10.1016/S0736-0266(01)00054-7
M3 - Article
C2 - 11781011
AN - SCOPUS:0035656898
SN - 0736-0266
VL - 19
SP - 1098
EP - 1104
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
IS - 6
ER -