Controlled heat stress promotes myofibrillogenesis during myogenesis

Qiongyu Guo, Devin Miller, Hongying An, Howard Wang, Joseph Lopez, Denver Lough, Ling He, Anand Kumar

Research output: Contribution to journalArticle

Abstract

Hyperthermia therapy has recently emerged as a clinical modality used to finely tune heat stress inside the human body for various biomedical applications. Nevertheless, little is known regarding the optimal timing or temperature of heat stress that is needed to achieve favorable results following hyperthermia therapy for muscle regeneration purposes. The regeneration of skeletal muscle after injury is a highly complex and coordinated process that involves a multitude of cellular mechanisms. The main objective of this study was to characterize the effects of hyperthermal therapy on the overall behavior of myoblasts during myogenic differentiation. Various cellular processes, including myogenesis, myofibrillogenesis, hypertrophy/atrophy, and mitochondrial biogenesis, were studied using systematic cellular, morphological, and pathway-focused high-throughput gene expression profiling analyses. We found that C2C12 myoblasts exhibited distinctive time and temperature-dependence in biosynthesis and regulatory events during myogenic differentiation. Specifically, we for the first time observed that moderate hyperthermia at 39°C favored the growth of sarcomere in myofibrils at the late stage of myogenesis, showing universal upregulation of characteristic myofibril proteins. Characteristic myofibrillogenesis genes, including heavy polypeptide 1 myosin, heavy polypeptide 2 myosin, alpha 1 actin, nebulin and titin, were all significantly upregulated (p<0.01) after C2C12 cells differentiated at 39°C over 5 days compared with the control cells cultured at 37?C. Furthermore, moderate hyperthermia enhanced myogenic differentiation, with nucleus densities per myotube showing 2.2-fold, 1.9-fold and 1.6-fold increases when C2C12 cells underwent myogenic differentiation at 39°C over 24 hours, 48 hours and 72 hours, respectively, as compared to the myotubes that were not exposed to heat stress. Yet, atrophy genes were sensitive even to moderate hyperthermia, indicating that strictly controlled heat stress is required to minimize the development of atrophy in myotubes. In addition, mitochondrial biogenesis was enhanced following thermal induction of myoblasts, suggesting a subsequent shift toward anabolic demand requirements for energy production. This study offers a new perspective to understand and utilize the time and temperature-sensitive effects of hyperthermal therapy on muscle regeneration.

Original languageEnglish (US)
Article numbere0166294
JournalPLoS One
Volume11
Issue number11
DOIs
StatePublished - Nov 1 2016

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Muscle Development
muscle development
heat stress
Fever
Hot Temperature
fever
Myoblasts
Skeletal Muscle Fibers
Hyperthermia therapy
myoblasts
Atrophy
Muscle
Regeneration
atrophy
Myofibrils
Organelle Biogenesis
Myosins
therapeutics
myofibrils
Temperature

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Guo, Q., Miller, D., An, H., Wang, H., Lopez, J., Lough, D., ... Kumar, A. (2016). Controlled heat stress promotes myofibrillogenesis during myogenesis. PLoS One, 11(11), [e0166294]. https://doi.org/10.1371/journal.pone.0166294

Controlled heat stress promotes myofibrillogenesis during myogenesis. / Guo, Qiongyu; Miller, Devin; An, Hongying; Wang, Howard; Lopez, Joseph; Lough, Denver; He, Ling; Kumar, Anand.

In: PLoS One, Vol. 11, No. 11, e0166294, 01.11.2016.

Research output: Contribution to journalArticle

Guo, Q, Miller, D, An, H, Wang, H, Lopez, J, Lough, D, He, L & Kumar, A 2016, 'Controlled heat stress promotes myofibrillogenesis during myogenesis', PLoS One, vol. 11, no. 11, e0166294. https://doi.org/10.1371/journal.pone.0166294
Guo Q, Miller D, An H, Wang H, Lopez J, Lough D et al. Controlled heat stress promotes myofibrillogenesis during myogenesis. PLoS One. 2016 Nov 1;11(11). e0166294. https://doi.org/10.1371/journal.pone.0166294
Guo, Qiongyu ; Miller, Devin ; An, Hongying ; Wang, Howard ; Lopez, Joseph ; Lough, Denver ; He, Ling ; Kumar, Anand. / Controlled heat stress promotes myofibrillogenesis during myogenesis. In: PLoS One. 2016 ; Vol. 11, No. 11.
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