Controlled drug delivery to the joints by enzymatically degradable microspheres

Kimberly E. Brown, Wen Shao, Joan Bathon, Kam W. Leong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An intra-articular polymeric controlled release system was developed that is tailored to, and responsive to, the intensity of joint inflammation. Microspheres composed of the naturally occurring polyelectrolytes, gelatin and chondroitin sulfate, were synthesized by complex coacervation and the kinetics of release of encapsulated 14C-catalase, was evaluated in vitro in the presence of inflammatory and non-inflammatory human joint fluids. The relative activity of gelatinase, a metalloprotease enzyme, was quantified in each of the joints fluids. Rate of degradation of microspheres, and consequent release of 14C-catalase, was found to parallel the relative gelatinase activities in the joint fluids. Furthermore, various methods of crosslinking were found to affect the kinetics of microsphere degradation in the fluids. A catalase loading level of up to 28% was achieved, and the encapsulated catalase was found to retain up to 58% of its biological activity.

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium Proceedings
PublisherPubl by Materials Research Society
Pages73-78
Number of pages6
Volume331
ISBN (Print)1558992308
StatePublished - 1994
EventProceedings of the Biomaterials for Drug and Cell Delivery - Boston, MA, USA
Duration: Nov 29 1993Dec 1 1993

Other

OtherProceedings of the Biomaterials for Drug and Cell Delivery
CityBoston, MA, USA
Period11/29/9312/1/93

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Engineering(all)
  • Earth and Planetary Sciences(all)
  • Environmental Science(all)

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