Control of fusion pore during exocytosis by Munc18

R. J. Fisher, J. Pevsner, R. D. Burgoyne

Research output: Contribution to journalArticle

Abstract

Intracellular membrane fusion is mediated by the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins. All vesicle transport Steps also have an essential requirement for a member of the Sec1 protein family, including the neuronal Munc18-1 (also known as nSec1) in regulated exocytosis. Here, in adrenal chromaffin cells, we expressed a Munc18 mutant with reduced affinity for syntaxin, which specifically modified the kinetics of single-granule exocytotic release events, consistent with an acceleration of fusion pore expansion. Thus, Munc18 functions in a late stage in the fusion process, where its dissociation from syntaxin determines the kinetics of postfusion events.

Original languageEnglish (US)
Pages (from-to)875-878
Number of pages4
JournalScience
Volume291
Issue number5505
DOIs
StatePublished - Feb 2 2001
Externally publishedYes

ASJC Scopus subject areas

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