Control of fecal peritoneal infection in mice by colony-stimulating factors

Johannes Barsig, Daniela Sophie Bundschuh, Thomas Hartung, Artur Bauhofer, Achim Sauer, Albrecht Wendel

Research output: Contribution to journalArticle

Abstract

Granulocyte colony-stimulating factor (G-CSF) recruits and primes neutrophilic granulocytes. The role of endogenous and exogenous G-CSF was examined in a murine fecal peritoneal infection model characterized by rapid production of high levels of circulating G-CSF. Pretreatment with anti- murine G-CSF for 5 days reduced neutrophil counts by 50% and sensitized mice to sublethal peritonitis. There were more aerobic bacteria in livers of antiserum-pretreated animals but fewer neutrophils in peritoneal cavities. Pretreatment with 100 μg/kg recombinant murine G-CSF intravenously for 2 days raised neutrophil counts 5-fold and significantly protected animals against lethal peritonitis. A similar prophylactic administration of murine granulocyte-macrophage (GM)-CSF neither augmented leukocyte numbers nor protected infected mice. These results show a dissociation between the pharmacologic properties of GM-CSF and G-CSF and demonstrate the crucial role of endogenous G-CSF in controlling neutrophil-dependent defense against bacterial invasion in infection.

Original languageEnglish (US)
Pages (from-to)790-799
Number of pages10
JournalJournal of Infectious Diseases
Volume174
Issue number4
StatePublished - 1996
Externally publishedYes

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Colony-Stimulating Factors
Granulocyte Colony-Stimulating Factor
Neutrophils
Infection
Granulocytes
Granulocyte-Macrophage Colony-Stimulating Factor
Peritonitis
Aerobic Bacteria
Peritoneal Cavity
Leukocyte Count
Immune Sera
Macrophages
Liver

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Barsig, J., Bundschuh, D. S., Hartung, T., Bauhofer, A., Sauer, A., & Wendel, A. (1996). Control of fecal peritoneal infection in mice by colony-stimulating factors. Journal of Infectious Diseases, 174(4), 790-799.

Control of fecal peritoneal infection in mice by colony-stimulating factors. / Barsig, Johannes; Bundschuh, Daniela Sophie; Hartung, Thomas; Bauhofer, Artur; Sauer, Achim; Wendel, Albrecht.

In: Journal of Infectious Diseases, Vol. 174, No. 4, 1996, p. 790-799.

Research output: Contribution to journalArticle

Barsig, J, Bundschuh, DS, Hartung, T, Bauhofer, A, Sauer, A & Wendel, A 1996, 'Control of fecal peritoneal infection in mice by colony-stimulating factors', Journal of Infectious Diseases, vol. 174, no. 4, pp. 790-799.
Barsig J, Bundschuh DS, Hartung T, Bauhofer A, Sauer A, Wendel A. Control of fecal peritoneal infection in mice by colony-stimulating factors. Journal of Infectious Diseases. 1996;174(4):790-799.
Barsig, Johannes ; Bundschuh, Daniela Sophie ; Hartung, Thomas ; Bauhofer, Artur ; Sauer, Achim ; Wendel, Albrecht. / Control of fecal peritoneal infection in mice by colony-stimulating factors. In: Journal of Infectious Diseases. 1996 ; Vol. 174, No. 4. pp. 790-799.
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