Control of cytoplasmic incompatibility in the Aedes scutellaris complex: Incompatible crosses become compatible by treatment of larvae with heat or antibiotics

M. Trpis, J. B. Perrone, M. Reissig, K. L. Parker

Research output: Contribution to journalArticle

Abstract

Most of the interspecific crosses in the Aedes scutellaris complex result in unidirectional cytoplasmic incompatibility. The case of cytoplasmic incompatibility presented here involves Aedes polynesiensis and Ae. kesseli. While the cross Ae. kesseli♀ and Ae. polynesiensis ♂ is compatible, the reciprocal cross is not. By treating the larval stages of the parental stock with heat or the antibiotics tetracycline hydrochloride and chloramphenicol, the incompatible cross Ae. polynesiensis ♀ × Ae. kesseli ♂ is made compatible. Both sexes of the hybrid progeny were fertile and no hybrid breakdown occurred in any subsequent generation tested up to F6. Presence of the rickettsia-type microorganisms (RMO) in the ovaries of both species and their absence from the treated parental strains and the hybrids may indicate that the RMO is responsible for this incompatibility. This is not proof of the cause of incompatibility, as the alteration of physiological functions and structure of the cytoplasmic organelles (mitochondria and ribosomes) of the mosquito (eukaryotic) cells by treatment with heat and antibiotics may be responsible for the alteration of incompatibility as well. Maintaining a temperature of 32.5°C, and treating larvae with tetracycline hydrochloride in a concentration of 0.1 g/l and chloramphenicol in a concentration of 2.0g/l was the most effective method for changing incompatible crosses into compatible ones in the Aedes scutellaris complex.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalThe Journal of heredity
Volume72
Issue number5
StatePublished - Sep 1981

Fingerprint

Aedes scutellaris
cytoplasmic incompatibility
Aedes polynesiensis
Aedes
Antibiotics
Microorganisms
Larva
Rickettsia
Hot Temperature
Heat
antibiotics
Chloramphenicol
Anti-Bacterial Agents
Tetracycline
heat
Mitochondria
larvae
chloramphenicol
tetracycline
Cytoplasmic Structures

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Genetics(clinical)
  • Biotechnology
  • Molecular Biology
  • Genetics
  • Agricultural and Biological Sciences(all)

Cite this

Control of cytoplasmic incompatibility in the Aedes scutellaris complex : Incompatible crosses become compatible by treatment of larvae with heat or antibiotics. / Trpis, M.; Perrone, J. B.; Reissig, M.; Parker, K. L.

In: The Journal of heredity, Vol. 72, No. 5, 09.1981, p. 313-317.

Research output: Contribution to journalArticle

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