Contribution of trafficking signals in the cytoplasmic tail of the infectious bronchitis virus spike protein to virus infection

Soonjeon Youn, Ellen W. Collisson, Carolyn E Machamer

Research output: Contribution to journalArticle

Abstract

Coronavirus spike (S) proteins are responsible for binding and fusion with target cells and thus play an essential role in virus infection. Recently, we identified a dilysine endoplasmic reticulum (ER) retrieval signal and a tyrosine-based endocytosis signal in the cytoplasmic tail of the S protein of infectious bronchitis virus (IBV). Here, an infectious cDNA clone of IBV was used to address the importance of the S protein trafficking signals to virus infection. We constructed infectious cDNA clones lacking the ER retrieval signal, the endocytosis signal, or both. The virus lacking the ER retrieval signal was viable. However, this virus had a growth defect at late times postinfection and produced larger plaques than IBV. Further analysis confirmed that the mutant S protein trafficked though the secretory pathway faster than wild-type S protein. A more dramatic phenotype was obtained when the endocytosis signal was mutated. Recombinant viruses lacking the endocytosis signal (in combination with a mutated dilysine signal or alone) could not be recovered, even though transient syncytia were formed in transfected cells. Our results suggest that the endocytosis signal of IBV S is essential for productive virus infection.

Original languageEnglish (US)
Pages (from-to)13209-13217
Number of pages9
JournalJournal of Virology
Volume79
Issue number21
DOIs
StatePublished - Nov 2005

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Infectious bronchitis virus
Virus Diseases
Endocytosis
endocytosis
tail
lysyllysine
viruses
Endoplasmic Reticulum
infection
endoplasmic reticulum
Protein S
Viruses
Proteins
proteins
Coronavirus Spike Glycoproteins
Complementary DNA
Clone Cells
clones
Secretory Pathway
Protein Transport

ASJC Scopus subject areas

  • Immunology

Cite this

Contribution of trafficking signals in the cytoplasmic tail of the infectious bronchitis virus spike protein to virus infection. / Youn, Soonjeon; Collisson, Ellen W.; Machamer, Carolyn E.

In: Journal of Virology, Vol. 79, No. 21, 11.2005, p. 13209-13217.

Research output: Contribution to journalArticle

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