Contribution of β-lactamases and porin proteins OmpK35 and OmpK36 to carbapenem resistance in clinical isolates of KPC-2-producing Klebsiella pneumoniae

Ying Zhang, Xiaofei Jiang, Yanyan Wang, Gang Li, Yueru Tian, Hong Liu, Fuqi Ai, Yiming Ma, Bei Wang, Feiyi Ruan, Kumar Rajakumar

Research output: Contribution to journalArticle

Abstract

Fifty-seven carbapenem-resistant Klebsiella pneumoniae isolates belonging to ST11 (50 isolates), ST423 (5 isolates), and two other sequence types were studied. All were positive for blaKPC-2, blaTEM-1, and blaCTX-M-14. SDS-PAGE analysis of six representative isolates demonstrated varied porin expression. Nevertheless, when blaKPC-2 was deleted, carbapenem resistance was markedly reduced. Additionally, SHV-12, DHA-1, and/or VIM-1 appeared to contribute to accessory carbapenemase activity. In contrast, OmpK35 and/or OmpK36 deficiency seemed to serve only as a minor cooperative factor.

Original languageEnglish (US)
Pages (from-to)1214-1217
Number of pages4
JournalAntimicrobial agents and chemotherapy
Volume58
Issue number2
DOIs
StatePublished - Feb 1 2014

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ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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