TY - JOUR
T1 - Contribution of β-lactamases and porin proteins OmpK35 and OmpK36 to carbapenem resistance in clinical isolates of KPC-2-producing Klebsiella pneumoniae
AU - Zhang, Ying
AU - Jiang, Xiaofei
AU - Wang, Yanyan
AU - Li, Gang
AU - Tian, Yueru
AU - Liu, Hong
AU - Ai, Fuqi
AU - Ma, Yiming
AU - Wang, Bei
AU - Ruan, Feiyi
AU - Rajakumar, Kumar
PY - 2014/2
Y1 - 2014/2
N2 - Fifty-seven carbapenem-resistant Klebsiella pneumoniae isolates belonging to ST11 (50 isolates), ST423 (5 isolates), and two other sequence types were studied. All were positive for blaKPC-2, blaTEM-1, and blaCTX-M-14. SDS-PAGE analysis of six representative isolates demonstrated varied porin expression. Nevertheless, when blaKPC-2 was deleted, carbapenem resistance was markedly reduced. Additionally, SHV-12, DHA-1, and/or VIM-1 appeared to contribute to accessory carbapenemase activity. In contrast, OmpK35 and/or OmpK36 deficiency seemed to serve only as a minor cooperative factor.
AB - Fifty-seven carbapenem-resistant Klebsiella pneumoniae isolates belonging to ST11 (50 isolates), ST423 (5 isolates), and two other sequence types were studied. All were positive for blaKPC-2, blaTEM-1, and blaCTX-M-14. SDS-PAGE analysis of six representative isolates demonstrated varied porin expression. Nevertheless, when blaKPC-2 was deleted, carbapenem resistance was markedly reduced. Additionally, SHV-12, DHA-1, and/or VIM-1 appeared to contribute to accessory carbapenemase activity. In contrast, OmpK35 and/or OmpK36 deficiency seemed to serve only as a minor cooperative factor.
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U2 - 10.1128/AAC.02045-12
DO - 10.1128/AAC.02045-12
M3 - Article
C2 - 24277031
AN - SCOPUS:84893472407
SN - 0066-4804
VL - 58
SP - 1214
EP - 1217
JO - Antimicrobial agents and chemotherapy
JF - Antimicrobial agents and chemotherapy
IS - 2
ER -