Contrasting antibody responses to intrasubtype superinfection with CRF02-AG

Colleen R. Courtney, Luzia Mayr, Aubin J. Nanfack, Andrew N. Banin, Michael Tuen, Ruimin Pan, Xunqing Jiang, Xiang Peng Kong, Allison R. Kirkpatrick, Daniel Bruno, Craig A. Martens, Lydia Sykora, Stephen F. Porcella, Andrew Redd, Thomas C Quinn, Phillipe N. Nyambi, Ralf Dürr

Research output: Contribution to journalArticle

Abstract

HIV superinfection describes the sequential infection of an individual with two or more unrelated HIV strains. Intersubtype superinfection has been shown to cause a broader and more potent heterologous neutralizing antibody response when compared to singly infected controls, yet the effects of intrasubtype superinfection remain controversial. Longitudinal samples were analyzed phylogenetically for pol and envregions using Next-Generation Sequencing and envelope cloning. The impact of CRF02-AG intrasubtype superinfection was assessed for heterologous neutralization and antibody binding responses. We compared two cases of CRF02-AG intrasubtype superinfection that revealed complete replacement of the initial virus by superinfecting CRF02-AG variants with signs of recombination. NYU6564, who became superinfected at an early time point, exhibited greater changes in antibody binding profiles and generated a more potent neutralizing antibody response post-superinfection compared to NYU6501. In contrast, superinfection occurred at a later time point in NYU6501 with strains harboring significantly longer V1V2 regions with no observable changes in neutralization patterns. Here we show that CRF02-AG intrasubtype superinfection can induce a cross-subtype neutralizing antibody response, and our data suggest timing and/or superinfecting viral envelope characteristics as contributing factors. These results highlight differential outcomes in intrasubtype superinfection and provide the first insight into cases with CRF02-AG, the fourth most prevalent HIV-1 strain worldwide.

Original languageEnglish (US)
Article numbere0173705
JournalPLoS One
Volume12
Issue number3
DOIs
StatePublished - Mar 1 2017

Fingerprint

Superinfection
Neutralizing Antibodies
Antibody Formation
antibodies
Antibodies
Heterophile Antibodies
Cloning
Viruses
neutralizing antibodies
neutralization
HIV
superinfection
Human immunodeficiency virus 1
Genetic Recombination
HIV-1
Organism Cloning
viruses

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Courtney, C. R., Mayr, L., Nanfack, A. J., Banin, A. N., Tuen, M., Pan, R., ... Dürr, R. (2017). Contrasting antibody responses to intrasubtype superinfection with CRF02-AG. PLoS One, 12(3), [e0173705]. https://doi.org/10.1371/journal.pone.0173705

Contrasting antibody responses to intrasubtype superinfection with CRF02-AG. / Courtney, Colleen R.; Mayr, Luzia; Nanfack, Aubin J.; Banin, Andrew N.; Tuen, Michael; Pan, Ruimin; Jiang, Xunqing; Kong, Xiang Peng; Kirkpatrick, Allison R.; Bruno, Daniel; Martens, Craig A.; Sykora, Lydia; Porcella, Stephen F.; Redd, Andrew; Quinn, Thomas C; Nyambi, Phillipe N.; Dürr, Ralf.

In: PLoS One, Vol. 12, No. 3, e0173705, 01.03.2017.

Research output: Contribution to journalArticle

Courtney, CR, Mayr, L, Nanfack, AJ, Banin, AN, Tuen, M, Pan, R, Jiang, X, Kong, XP, Kirkpatrick, AR, Bruno, D, Martens, CA, Sykora, L, Porcella, SF, Redd, A, Quinn, TC, Nyambi, PN & Dürr, R 2017, 'Contrasting antibody responses to intrasubtype superinfection with CRF02-AG', PLoS One, vol. 12, no. 3, e0173705. https://doi.org/10.1371/journal.pone.0173705
Courtney CR, Mayr L, Nanfack AJ, Banin AN, Tuen M, Pan R et al. Contrasting antibody responses to intrasubtype superinfection with CRF02-AG. PLoS One. 2017 Mar 1;12(3). e0173705. https://doi.org/10.1371/journal.pone.0173705
Courtney, Colleen R. ; Mayr, Luzia ; Nanfack, Aubin J. ; Banin, Andrew N. ; Tuen, Michael ; Pan, Ruimin ; Jiang, Xunqing ; Kong, Xiang Peng ; Kirkpatrick, Allison R. ; Bruno, Daniel ; Martens, Craig A. ; Sykora, Lydia ; Porcella, Stephen F. ; Redd, Andrew ; Quinn, Thomas C ; Nyambi, Phillipe N. ; Dürr, Ralf. / Contrasting antibody responses to intrasubtype superinfection with CRF02-AG. In: PLoS One. 2017 ; Vol. 12, No. 3.
@article{6f2b4fce9f1849fcb344936c1fde557b,
title = "Contrasting antibody responses to intrasubtype superinfection with CRF02-AG",
abstract = "HIV superinfection describes the sequential infection of an individual with two or more unrelated HIV strains. Intersubtype superinfection has been shown to cause a broader and more potent heterologous neutralizing antibody response when compared to singly infected controls, yet the effects of intrasubtype superinfection remain controversial. Longitudinal samples were analyzed phylogenetically for pol and envregions using Next-Generation Sequencing and envelope cloning. The impact of CRF02-AG intrasubtype superinfection was assessed for heterologous neutralization and antibody binding responses. We compared two cases of CRF02-AG intrasubtype superinfection that revealed complete replacement of the initial virus by superinfecting CRF02-AG variants with signs of recombination. NYU6564, who became superinfected at an early time point, exhibited greater changes in antibody binding profiles and generated a more potent neutralizing antibody response post-superinfection compared to NYU6501. In contrast, superinfection occurred at a later time point in NYU6501 with strains harboring significantly longer V1V2 regions with no observable changes in neutralization patterns. Here we show that CRF02-AG intrasubtype superinfection can induce a cross-subtype neutralizing antibody response, and our data suggest timing and/or superinfecting viral envelope characteristics as contributing factors. These results highlight differential outcomes in intrasubtype superinfection and provide the first insight into cases with CRF02-AG, the fourth most prevalent HIV-1 strain worldwide.",
author = "Courtney, {Colleen R.} and Luzia Mayr and Nanfack, {Aubin J.} and Banin, {Andrew N.} and Michael Tuen and Ruimin Pan and Xunqing Jiang and Kong, {Xiang Peng} and Kirkpatrick, {Allison R.} and Daniel Bruno and Martens, {Craig A.} and Lydia Sykora and Porcella, {Stephen F.} and Andrew Redd and Quinn, {Thomas C} and Nyambi, {Phillipe N.} and Ralf D{\"u}rr",
year = "2017",
month = "3",
day = "1",
doi = "10.1371/journal.pone.0173705",
language = "English (US)",
volume = "12",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

TY - JOUR

T1 - Contrasting antibody responses to intrasubtype superinfection with CRF02-AG

AU - Courtney, Colleen R.

AU - Mayr, Luzia

AU - Nanfack, Aubin J.

AU - Banin, Andrew N.

AU - Tuen, Michael

AU - Pan, Ruimin

AU - Jiang, Xunqing

AU - Kong, Xiang Peng

AU - Kirkpatrick, Allison R.

AU - Bruno, Daniel

AU - Martens, Craig A.

AU - Sykora, Lydia

AU - Porcella, Stephen F.

AU - Redd, Andrew

AU - Quinn, Thomas C

AU - Nyambi, Phillipe N.

AU - Dürr, Ralf

PY - 2017/3/1

Y1 - 2017/3/1

N2 - HIV superinfection describes the sequential infection of an individual with two or more unrelated HIV strains. Intersubtype superinfection has been shown to cause a broader and more potent heterologous neutralizing antibody response when compared to singly infected controls, yet the effects of intrasubtype superinfection remain controversial. Longitudinal samples were analyzed phylogenetically for pol and envregions using Next-Generation Sequencing and envelope cloning. The impact of CRF02-AG intrasubtype superinfection was assessed for heterologous neutralization and antibody binding responses. We compared two cases of CRF02-AG intrasubtype superinfection that revealed complete replacement of the initial virus by superinfecting CRF02-AG variants with signs of recombination. NYU6564, who became superinfected at an early time point, exhibited greater changes in antibody binding profiles and generated a more potent neutralizing antibody response post-superinfection compared to NYU6501. In contrast, superinfection occurred at a later time point in NYU6501 with strains harboring significantly longer V1V2 regions with no observable changes in neutralization patterns. Here we show that CRF02-AG intrasubtype superinfection can induce a cross-subtype neutralizing antibody response, and our data suggest timing and/or superinfecting viral envelope characteristics as contributing factors. These results highlight differential outcomes in intrasubtype superinfection and provide the first insight into cases with CRF02-AG, the fourth most prevalent HIV-1 strain worldwide.

AB - HIV superinfection describes the sequential infection of an individual with two or more unrelated HIV strains. Intersubtype superinfection has been shown to cause a broader and more potent heterologous neutralizing antibody response when compared to singly infected controls, yet the effects of intrasubtype superinfection remain controversial. Longitudinal samples were analyzed phylogenetically for pol and envregions using Next-Generation Sequencing and envelope cloning. The impact of CRF02-AG intrasubtype superinfection was assessed for heterologous neutralization and antibody binding responses. We compared two cases of CRF02-AG intrasubtype superinfection that revealed complete replacement of the initial virus by superinfecting CRF02-AG variants with signs of recombination. NYU6564, who became superinfected at an early time point, exhibited greater changes in antibody binding profiles and generated a more potent neutralizing antibody response post-superinfection compared to NYU6501. In contrast, superinfection occurred at a later time point in NYU6501 with strains harboring significantly longer V1V2 regions with no observable changes in neutralization patterns. Here we show that CRF02-AG intrasubtype superinfection can induce a cross-subtype neutralizing antibody response, and our data suggest timing and/or superinfecting viral envelope characteristics as contributing factors. These results highlight differential outcomes in intrasubtype superinfection and provide the first insight into cases with CRF02-AG, the fourth most prevalent HIV-1 strain worldwide.

UR - http://www.scopus.com/inward/record.url?scp=85015345194&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015345194&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0173705

DO - 10.1371/journal.pone.0173705

M3 - Article

VL - 12

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 3

M1 - e0173705

ER -