Contractile dysfunction and ATP depletion after transient calcium overload in perfused ferret hearts

M. Kitakaze, H. F. Weisman, E. Marban

Research output: Contribution to journalArticle

Abstract

Although a number of lines of evidence hint that an elevation of intracellular calcium leads to myocardial injury, the cellular consequences of transient Ca overload remain unclear. To determine the contractile, histologic, and metabolic sequelae of transient Ca overload, we measured developed pressure (DP) in isovolumetric Langendorff-perfused ferret hearts at 37°C before and 20 min after three 5 min periods of perfusion with a 10 mM [Ca]0, 1 mM [Mg]0 solution (high-Ca group, n = 8) without ischemia, and in control hearts (n = 5) exposed transiently to the same total divalent cation concentration without a change in [Ca]0 (9 mM [Mg]0, 2 mM [Ca]0). DP, measured at various [Ca]0 (0.5 to 5 mM), was depressed in the high-Ca group relative to control (p <.001). Representative hearts from the control group were histologically normal, whereas hearts from the high-Ca group exhibited rare foci of predominantly 'reversible' injury (mitochondrial swelling, glycogen deposition, and clumping of nuclear chromatin). Maximal Ca++-activated pressure (MCAP), measured from tetani after exposure to ryanodine, was also decreased in the high-Ca group (230 ± 4 vs 262 ± 6 mm Hg, p <.001). Ca0 sensitivity, determined by normalization of the DP-[Ca]0 relationship to the corresponding MCAP, was shifted to higher [Ca]0 in the high-Ca group. Phosphorus nuclear magnetic resonance spectra were obtained in four high-Ca hearts. [ATP] declined by 30% to 40% after exposure to high [Ca]0, but inorganic phosphate, phosphocreatine, and pH remained unchanged. These results indicate that transient exposure to high [Ca]0 without ischemia leaves behind distinctive contractile, metabolic, and histologic sequelae. The possible implications for the pathogenesis of postischemic contractile dysfunction are discussed.

Original languageEnglish (US)
Pages (from-to)685-695
Number of pages11
JournalCirculation
Volume77
Issue number3
StatePublished - 1988

Fingerprint

Ferrets
Adenosine Triphosphate
Calcium
Pressure
Ischemia
Mitochondrial Swelling
Ryanodine
Phosphocreatine
Divalent Cations
Wounds and Injuries
Tetanus
Glycogen
Phosphorus
Chromatin
Magnetic Resonance Spectroscopy
Perfusion
Phosphates
Control Groups

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Contractile dysfunction and ATP depletion after transient calcium overload in perfused ferret hearts. / Kitakaze, M.; Weisman, H. F.; Marban, E.

In: Circulation, Vol. 77, No. 3, 1988, p. 685-695.

Research output: Contribution to journalArticle

Kitakaze, M, Weisman, HF & Marban, E 1988, 'Contractile dysfunction and ATP depletion after transient calcium overload in perfused ferret hearts', Circulation, vol. 77, no. 3, pp. 685-695.
Kitakaze, M. ; Weisman, H. F. ; Marban, E. / Contractile dysfunction and ATP depletion after transient calcium overload in perfused ferret hearts. In: Circulation. 1988 ; Vol. 77, No. 3. pp. 685-695.
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