Construction of comprehensive dosage-matching core histone mutant libraries for Saccharomyces cerevisiae

Shuangying Jiang, Yan Liu, Ann Wang, Yiran Qin, Maoguo Luo, Qingyu Wu, Jef D. Boeke, Junbiao Dai

Research output: Contribution to journalArticle

Abstract

Saccharomyces cerevisiae contains two genes for each core histone, which are presented as pairs under the control of a divergent promoter, i.e., HHT1-HHF1, HHT2-HHF2, HTA1-HTB1 and HTA2-HTB2. HHT1-HHF1, and HHT2-HHF2 encode histone H3 and H4 with identical amino acid sequences but under the control of differently regulated promoters. Previous mutagenesis studies were carried out by deleting one pair and mutating the other one. Here, we present the design and construction of three additional libraries covering HTA1-HTB1, HTA2-HTB2, and HHT1-HHF1 respectively. Together with the previously described library of HHT2-HHF2 mutants, a systematic and complete collection of mutants for each of the eight core S. cerevisiae histone genes becomes available. Each designed mutant was incorporated into the genome, generating three more corresponding libraries of yeast strains. We demonstrated that, although, under normal growth conditions, strains with single-copy integrated histone genes lacked phenotypes, in some growth conditions, growth deficiencies were observed. Specifically, we showed that addition of a second copy of the mutant histone gene could rescue the lethality in some previously known mutants that cannot survive with a single copy. This resource enables systematic studies of function of each nucleosome residue in plasmid, single-copy, and double-copy integrated formats.

Original languageEnglish (US)
Pages (from-to)1263-1273
Number of pages11
JournalGenetics
Volume207
Issue number4
DOIs
StatePublished - Dec 1 2017

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Histones
Saccharomyces cerevisiae
Libraries
Genes
Growth
Nucleosomes
Mutagenesis
Amino Acid Sequence
Plasmids
Yeasts
Genome
Phenotype

Keywords

  • Histone H2A
  • Histone H2B
  • Histone H3
  • Histone H4
  • Mutagenesis

ASJC Scopus subject areas

  • Genetics

Cite this

Construction of comprehensive dosage-matching core histone mutant libraries for Saccharomyces cerevisiae. / Jiang, Shuangying; Liu, Yan; Wang, Ann; Qin, Yiran; Luo, Maoguo; Wu, Qingyu; Boeke, Jef D.; Dai, Junbiao.

In: Genetics, Vol. 207, No. 4, 01.12.2017, p. 1263-1273.

Research output: Contribution to journalArticle

Jiang, S, Liu, Y, Wang, A, Qin, Y, Luo, M, Wu, Q, Boeke, JD & Dai, J 2017, 'Construction of comprehensive dosage-matching core histone mutant libraries for Saccharomyces cerevisiae', Genetics, vol. 207, no. 4, pp. 1263-1273. https://doi.org/10.1534/genetics.117.300450
Jiang, Shuangying ; Liu, Yan ; Wang, Ann ; Qin, Yiran ; Luo, Maoguo ; Wu, Qingyu ; Boeke, Jef D. ; Dai, Junbiao. / Construction of comprehensive dosage-matching core histone mutant libraries for Saccharomyces cerevisiae. In: Genetics. 2017 ; Vol. 207, No. 4. pp. 1263-1273.
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