Construction of chicken x human microcell hybrids for human gene targeting

M. Koi, P. W. Lamb, L. Filatov, Andrew P Feinberg, J. C. Barrett

Research output: Contribution to journalArticle

Abstract

Human chromosomes 1, 2, 3, and 11 were tagged with pSV2 neo and transferred via microcell fusion from mouse A9 human monochromosomal hybrids to a chicken pre-B cell line, DT40, proficient for homologous recombination. Hybrids containing two copies of human chromosome 11 were transfected with targeting vectors containing a mammalian selectable gene with either the D11S16 or HRAS genomic sequences corresponding to two different chromosome 11 loci. Analysis of stable transfectants showed a high frequency (~ 80%) of targeted integration of these constructs into each of the homologous loci of human chromosome 11 in DT40 hybrids. The results suggest that any human genomic sequences on human chromosomes transferred into DT40 cells could be targeted at high frequency, thereby allowing for subsequent modification of human genes and chromosomes.

Original languageEnglish (US)
Pages (from-to)72-76
Number of pages5
JournalCytogenetics and Cell Genetics
Volume76
Issue number1-2
StatePublished - 1997

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Gene Targeting
Human Chromosomes
Chromosomes, Human, Pair 11
Chickens
Chromosomes, Human, 1-3
B-Lymphoid Precursor Cells
Homologous Recombination
Genes
Cell Line

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Construction of chicken x human microcell hybrids for human gene targeting. / Koi, M.; Lamb, P. W.; Filatov, L.; Feinberg, Andrew P; Barrett, J. C.

In: Cytogenetics and Cell Genetics, Vol. 76, No. 1-2, 1997, p. 72-76.

Research output: Contribution to journalArticle

Koi, M, Lamb, PW, Filatov, L, Feinberg, AP & Barrett, JC 1997, 'Construction of chicken x human microcell hybrids for human gene targeting', Cytogenetics and Cell Genetics, vol. 76, no. 1-2, pp. 72-76.
Koi, M. ; Lamb, P. W. ; Filatov, L. ; Feinberg, Andrew P ; Barrett, J. C. / Construction of chicken x human microcell hybrids for human gene targeting. In: Cytogenetics and Cell Genetics. 1997 ; Vol. 76, No. 1-2. pp. 72-76.
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