Construction of a new bacterial cloning vector using a mutant green fluorescent protein as an indicator

Yue Mei Dong, Jiu Di Li, Zhi Qing Zhu

Research output: Contribution to journalArticle

Abstract

A new bacterial cloning vector, pGreenLD, derived from the triple substitution mutated Aequorea victoria green fluorescent protein (GFP-S65A, V68L, S72A), when expressed in E. coli produced colonies which showed yellow-green colour under daylight and strong green fluorescence under long-wave ultraviolet light. It can be a useful vector for selecting foreign DNA fragment which was inserted into multiple cloning site based on the loss of the yellow-green color/green fluorescence of E. coli cells attributable to the insertional inactivation of GFP production.

Original languageEnglish (US)
Pages (from-to)487-489
Number of pages3
JournalActa Botanica Sinica
Volume41
Issue number5
StatePublished - May 1 1999
Externally publishedYes

Keywords

  • Cloning vector
  • GFPmut2
  • Green fluorescent protein
  • pBluescript SK(+)

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

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