Construction of a map of chromosome 16 by using radiation hybrids

I. Ceccherini, G. Romeo, S. Lawrence, M. H. Breuning, P. C. Harris, H. Himmelbauer, A. M. Frischauf, G. R. Sutherland, G. G. Germino, S. T. Reeders, N. E. Morton

Research output: Contribution to journalArticle

Abstract

A human-hamster cell hybrid carrying a single copy of chromosome 16 as the only human genetic material was irradiated with a single dose of γ-rays (7000 rads; 1 rad = 0.01 Gy) and then fused with a thymidine kinase-deficient hamster cell line (RJKM) to generate radiation hybrids retaining unselected fragments of this human chromosome. In two experiments, 223 hybrids were isolated in hypoxanthine/aminopterine/thymidine (HAT) medium and screened with 38 DNA probes, corresponding to anonymous DNA or gene sequences localized on chromosome 16. The most likely order and location of the 38 DNA sequences were established by multiple pairwise analysis and scaled to estimate physical distance in megabases. The order and the distances thus obtained are mostly consistent with available data on genetic and physical mapping of these markers, illustrating the usefulness of radiation hybrids for mapping.

Original languageEnglish (US)
Pages (from-to)104-108
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number1
StatePublished - 1992
Externally publishedYes

Keywords

  • Multiple pairwise analysis
  • Physical maps
  • Somatic cell hybrids

ASJC Scopus subject areas

  • Genetics
  • General

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  • Cite this

    Ceccherini, I., Romeo, G., Lawrence, S., Breuning, M. H., Harris, P. C., Himmelbauer, H., Frischauf, A. M., Sutherland, G. R., Germino, G. G., Reeders, S. T., & Morton, N. E. (1992). Construction of a map of chromosome 16 by using radiation hybrids. Proceedings of the National Academy of Sciences of the United States of America, 89(1), 104-108.