Constructing deletions with defined endpoints in Drosophila

Lynn Cooley, Dianne Thompson, Allan C. Spradling

Research output: Contribution to journalArticlepeer-review

Abstract

Chromosomes bearing small deletions are valuable tools in Drosophila genetics. We have investigated a method for efficiently constructing precise chromosomal deficiencies. Two P transposable elements were positioned within a progenitor strain at the sites of the desired deletion endpoints. Deletions spanning the two transposons were recovered at high frequency when P element transposase was expressed in these flies, but only if the flanking P elements were in a cis rather than a trans configuration. Appropriate progenitor strains can now be constructed to delete virtually any chromosomal region by utilizing an extensive collection of lines containing single P element insertions throughout the Drosophila genome.

Original languageEnglish (US)
Pages (from-to)3170-3173
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number8
StatePublished - Apr 1990
Externally publishedYes

Keywords

  • Deficiency
  • Ebony gene
  • P element
  • Transposase

ASJC Scopus subject areas

  • Genetics
  • General

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