Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus

Ambroise Lambert, Aster Vanhecke, Anna Archetti, Seamus Holden, Felix Schaber, Zachary Pincus, Michael T. Laub, Erin D Goley, Suliana Manley

Research output: Contribution to journalArticle

Abstract

Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls and then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin ([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid)inhibition. We report that the constriction rate contributes to both size control and homeostasis, by determining elongation during constriction and by compensating for variation in pre-constriction elongation on a single-cell basis.

Original languageEnglish (US)
Pages (from-to)180-189
Number of pages10
JournalFood Science and Human Wellness
Volume4
DOIs
StatePublished - Jun 29 2018

Fingerprint

Cell Size
Constriction
homeostasis
Homeostasis
cell cycle
Caulobacter crescentus
phosphorous acid
cells
lighting
cell division
cell growth
microscopy
Cell Cycle
mutants
genetic markers
Fosfomycin
bacteria
Lighting
Cell Division
Microscopy

Keywords

  • Microbial Cell Structure
  • Microbial Physiology
  • Microbiology

ASJC Scopus subject areas

  • Food Science
  • General

Cite this

Lambert, A., Vanhecke, A., Archetti, A., Holden, S., Schaber, F., Pincus, Z., ... Manley, S. (2018). Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus. Food Science and Human Wellness, 4, 180-189. https://doi.org/10.1016/j.isci.2018.05.020

Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus. / Lambert, Ambroise; Vanhecke, Aster; Archetti, Anna; Holden, Seamus; Schaber, Felix; Pincus, Zachary; Laub, Michael T.; Goley, Erin D; Manley, Suliana.

In: Food Science and Human Wellness, Vol. 4, 29.06.2018, p. 180-189.

Research output: Contribution to journalArticle

Lambert, A, Vanhecke, A, Archetti, A, Holden, S, Schaber, F, Pincus, Z, Laub, MT, Goley, ED & Manley, S 2018, 'Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus', Food Science and Human Wellness, vol. 4, pp. 180-189. https://doi.org/10.1016/j.isci.2018.05.020
Lambert, Ambroise ; Vanhecke, Aster ; Archetti, Anna ; Holden, Seamus ; Schaber, Felix ; Pincus, Zachary ; Laub, Michael T. ; Goley, Erin D ; Manley, Suliana. / Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus. In: Food Science and Human Wellness. 2018 ; Vol. 4. pp. 180-189.
@article{b536505b7db341fda94efff6ba8538f0,
title = "Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus",
abstract = "Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls and then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin ([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid)inhibition. We report that the constriction rate contributes to both size control and homeostasis, by determining elongation during constriction and by compensating for variation in pre-constriction elongation on a single-cell basis.",
keywords = "Microbial Cell Structure, Microbial Physiology, Microbiology",
author = "Ambroise Lambert and Aster Vanhecke and Anna Archetti and Seamus Holden and Felix Schaber and Zachary Pincus and Laub, {Michael T.} and Goley, {Erin D} and Suliana Manley",
year = "2018",
month = "6",
day = "29",
doi = "10.1016/j.isci.2018.05.020",
language = "English (US)",
volume = "4",
pages = "180--189",
journal = "Food Science and Human Wellness",
issn = "2213-4530",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus

AU - Lambert, Ambroise

AU - Vanhecke, Aster

AU - Archetti, Anna

AU - Holden, Seamus

AU - Schaber, Felix

AU - Pincus, Zachary

AU - Laub, Michael T.

AU - Goley, Erin D

AU - Manley, Suliana

PY - 2018/6/29

Y1 - 2018/6/29

N2 - Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls and then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin ([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid)inhibition. We report that the constriction rate contributes to both size control and homeostasis, by determining elongation during constriction and by compensating for variation in pre-constriction elongation on a single-cell basis.

AB - Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls and then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin ([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid)inhibition. We report that the constriction rate contributes to both size control and homeostasis, by determining elongation during constriction and by compensating for variation in pre-constriction elongation on a single-cell basis.

KW - Microbial Cell Structure

KW - Microbial Physiology

KW - Microbiology

UR - http://www.scopus.com/inward/record.url?scp=85064940157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064940157&partnerID=8YFLogxK

U2 - 10.1016/j.isci.2018.05.020

DO - 10.1016/j.isci.2018.05.020

M3 - Article

C2 - 30240739

AN - SCOPUS:85064940157

VL - 4

SP - 180

EP - 189

JO - Food Science and Human Wellness

JF - Food Science and Human Wellness

SN - 2213-4530

ER -