Constitutive transcriptional activation by a β-catenin-Tcf complex in APC(-/-) colon carcinoma

Vladimir Korinek, Nick Barker, Patrice J. Morin, Dick Van Wichen, Roel De Weger, Kenneth W Kinzler, Bert Vogelstein, Hans Clevers

Research output: Contribution to journalArticle

Abstract

The adenomatous polyposis coli (APC) tumor suppressor protein binds to β-catenin, a protein recently shown to interact with Tcf and Lef transcription factors. The gene encoding hTcf-4, a Tcf family member that is expressed in colonic epithelium, was cloned and characterized. hTcf-4 transactivates transcription only when associated with β-catenin. Nuclei of APC(-/-) colon carcinoma cells were found to contain a stable β-catenin hTcf-4 complex that was constitutively active, as measured by transcription of a Tcf reporter gene. Reintroduction of APC removed β-catenin from hTcf-4 and abrogated the transcriptional transactivation. Constitutive transcription of Tcf target genes, caused by loss of APC function, may be a crucial event in the early transformation of colonic epithelium.

Original languageEnglish (US)
Pages (from-to)1784-1787
Number of pages4
JournalScience
Volume275
Issue number5307
DOIs
StatePublished - Mar 21 1997
Externally publishedYes

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Catenins
Adenomatous Polyposis Coli
Transcriptional Activation
Carcinoma
Adenomatous Polyposis Coli Protein
Epithelium
Tumor Suppressor Proteins
Reporter Genes
Genes
Transcription Factors

ASJC Scopus subject areas

  • General

Cite this

Korinek, V., Barker, N., Morin, P. J., Van Wichen, D., De Weger, R., Kinzler, K. W., ... Clevers, H. (1997). Constitutive transcriptional activation by a β-catenin-Tcf complex in APC(-/-) colon carcinoma. Science, 275(5307), 1784-1787. https://doi.org/10.1126/science.275.5307.1784

Constitutive transcriptional activation by a β-catenin-Tcf complex in APC(-/-) colon carcinoma. / Korinek, Vladimir; Barker, Nick; Morin, Patrice J.; Van Wichen, Dick; De Weger, Roel; Kinzler, Kenneth W; Vogelstein, Bert; Clevers, Hans.

In: Science, Vol. 275, No. 5307, 21.03.1997, p. 1784-1787.

Research output: Contribution to journalArticle

Korinek, V, Barker, N, Morin, PJ, Van Wichen, D, De Weger, R, Kinzler, KW, Vogelstein, B & Clevers, H 1997, 'Constitutive transcriptional activation by a β-catenin-Tcf complex in APC(-/-) colon carcinoma', Science, vol. 275, no. 5307, pp. 1784-1787. https://doi.org/10.1126/science.275.5307.1784
Korinek, Vladimir ; Barker, Nick ; Morin, Patrice J. ; Van Wichen, Dick ; De Weger, Roel ; Kinzler, Kenneth W ; Vogelstein, Bert ; Clevers, Hans. / Constitutive transcriptional activation by a β-catenin-Tcf complex in APC(-/-) colon carcinoma. In: Science. 1997 ; Vol. 275, No. 5307. pp. 1784-1787.
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