Constitutional and somatic rearrangement of chromosome 21 in acute lymphoblastic leukaemia

Yilong Li, Claire Schwab, Sarra L. Ryan, Elli Papaemmanuil, Hazel M. Robinson, Patricia Jacobs, Anthony Moorman, Sara Dyer, Julian Borrow, Mike Griffiths, Nyla A. Heerema, Andrew J. Carroll, Polly Talley, Nick Bown, Nick Telford, Fiona M. Ross, Lorraine Gaunt, Richard J.Q. McNally, Bryan D. Young, Paul SinclairVikki Rand, Manuel R. Teixeira, Olivia Joseph, Ben Robinson, Mark Maddison, Nicole Dastugue, Peter Vandenberghe, Claudia Haferlach, Philip J. Stephens, Jiqiu Cheng, Peter Van Loo, Michael R. Stratton, Peter J. Campbell, Christine J. Harrison

Research output: Contribution to journalArticle

Abstract

Changes in gene dosage are a major driver of cancer, known to be caused by a finite, but increasingly well annotated, repertoire of mutational mechanisms. This can potentially generate correlated copy-number alterations across hundreds of linked genes, as exemplified by the 2% of childhood acute lymphoblastic leukaemia (ALL) with recurrent amplification of megabase regions of chromosome 21 (iAMP21). We used genomic, cytogenetic and transcriptional analysis, coupled with novel bioinformatic approaches, to reconstruct the evolution of iAMP21 ALL. Here we show that individuals born with the rare constitutional Robertsonian translocation between chromosomes 15 and 21, rob(15;21)(q10;q10)c, have approximately 2,700-fold increased risk of developing iAMP21 ALL compared to the general population. In such cases, amplification is initiated by a chromothripsis event involving both sister chromatids of the Robertsonian chromosome, a novel mechanism for cancer predisposition. In sporadic iAMP21, breakage-fusion-bridge cycles are typically the initiating event, often followed by chromothripsis. In both sporadic and rob(15;21)c-associated iAMP21, the final stages frequently involve duplications of the entire abnormal chromosome. The end-product is a derivative of chromosome 21 or the rob(15;21)c chromosome with gene dosage optimized for leukaemic potential, showing constrained copy-number levels over multiple linked genes. Thus, dicentric chromosomes may be an important precipitant of chromothripsis, as we show rob(15;21)c to be constitutionally dicentric and breakage-fusion-bridge cycles generate dicentric chromosomes somatically. Furthermore, our data illustrate that several cancer-specific mutational processes, applied sequentially, can coordinate to fashion copy-number profiles over large genomic scales, incrementally refining the fitness benefits of aggregated gene dosage changes.

Original languageEnglish (US)
Pages (from-to)98-102
Number of pages5
JournalNature
Volume508
Issue number1
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Chromosomes, Human, Pair 21
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Gene Dosage
Chromosomes
Chromosomes, Human, Pair 15
Neoplasms
Chromatids
Cytogenetic Analysis
Computational Biology
Genes
Population
Chromothripsis

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Li, Y., Schwab, C., Ryan, S. L., Papaemmanuil, E., Robinson, H. M., Jacobs, P., ... Harrison, C. J. (2014). Constitutional and somatic rearrangement of chromosome 21 in acute lymphoblastic leukaemia. Nature, 508(1), 98-102. https://doi.org/10.1038/nature13115

Constitutional and somatic rearrangement of chromosome 21 in acute lymphoblastic leukaemia. / Li, Yilong; Schwab, Claire; Ryan, Sarra L.; Papaemmanuil, Elli; Robinson, Hazel M.; Jacobs, Patricia; Moorman, Anthony; Dyer, Sara; Borrow, Julian; Griffiths, Mike; Heerema, Nyla A.; Carroll, Andrew J.; Talley, Polly; Bown, Nick; Telford, Nick; Ross, Fiona M.; Gaunt, Lorraine; McNally, Richard J.Q.; Young, Bryan D.; Sinclair, Paul; Rand, Vikki; Teixeira, Manuel R.; Joseph, Olivia; Robinson, Ben; Maddison, Mark; Dastugue, Nicole; Vandenberghe, Peter; Haferlach, Claudia; Stephens, Philip J.; Cheng, Jiqiu; Van Loo, Peter; Stratton, Michael R.; Campbell, Peter J.; Harrison, Christine J.

In: Nature, Vol. 508, No. 1, 01.01.2014, p. 98-102.

Research output: Contribution to journalArticle

Li, Y, Schwab, C, Ryan, SL, Papaemmanuil, E, Robinson, HM, Jacobs, P, Moorman, A, Dyer, S, Borrow, J, Griffiths, M, Heerema, NA, Carroll, AJ, Talley, P, Bown, N, Telford, N, Ross, FM, Gaunt, L, McNally, RJQ, Young, BD, Sinclair, P, Rand, V, Teixeira, MR, Joseph, O, Robinson, B, Maddison, M, Dastugue, N, Vandenberghe, P, Haferlach, C, Stephens, PJ, Cheng, J, Van Loo, P, Stratton, MR, Campbell, PJ & Harrison, CJ 2014, 'Constitutional and somatic rearrangement of chromosome 21 in acute lymphoblastic leukaemia', Nature, vol. 508, no. 1, pp. 98-102. https://doi.org/10.1038/nature13115
Li Y, Schwab C, Ryan SL, Papaemmanuil E, Robinson HM, Jacobs P et al. Constitutional and somatic rearrangement of chromosome 21 in acute lymphoblastic leukaemia. Nature. 2014 Jan 1;508(1):98-102. https://doi.org/10.1038/nature13115
Li, Yilong ; Schwab, Claire ; Ryan, Sarra L. ; Papaemmanuil, Elli ; Robinson, Hazel M. ; Jacobs, Patricia ; Moorman, Anthony ; Dyer, Sara ; Borrow, Julian ; Griffiths, Mike ; Heerema, Nyla A. ; Carroll, Andrew J. ; Talley, Polly ; Bown, Nick ; Telford, Nick ; Ross, Fiona M. ; Gaunt, Lorraine ; McNally, Richard J.Q. ; Young, Bryan D. ; Sinclair, Paul ; Rand, Vikki ; Teixeira, Manuel R. ; Joseph, Olivia ; Robinson, Ben ; Maddison, Mark ; Dastugue, Nicole ; Vandenberghe, Peter ; Haferlach, Claudia ; Stephens, Philip J. ; Cheng, Jiqiu ; Van Loo, Peter ; Stratton, Michael R. ; Campbell, Peter J. ; Harrison, Christine J. / Constitutional and somatic rearrangement of chromosome 21 in acute lymphoblastic leukaemia. In: Nature. 2014 ; Vol. 508, No. 1. pp. 98-102.
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AU - Li, Yilong

AU - Schwab, Claire

AU - Ryan, Sarra L.

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AU - Jacobs, Patricia

AU - Moorman, Anthony

AU - Dyer, Sara

AU - Borrow, Julian

AU - Griffiths, Mike

AU - Heerema, Nyla A.

AU - Carroll, Andrew J.

AU - Talley, Polly

AU - Bown, Nick

AU - Telford, Nick

AU - Ross, Fiona M.

AU - Gaunt, Lorraine

AU - McNally, Richard J.Q.

AU - Young, Bryan D.

AU - Sinclair, Paul

AU - Rand, Vikki

AU - Teixeira, Manuel R.

AU - Joseph, Olivia

AU - Robinson, Ben

AU - Maddison, Mark

AU - Dastugue, Nicole

AU - Vandenberghe, Peter

AU - Haferlach, Claudia

AU - Stephens, Philip J.

AU - Cheng, Jiqiu

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