Consil® bioactive glass particles enhance osteoblast proliferation and selectively modulate cell signaling pathways in vitro

Angela Y. Au, Rebecca Y. Au, Jennifer L. Demko, Ron M. McLaughlin, Barbara E. Eves, Carmelita G. Frondoza

Research output: Contribution to journalArticle

Abstract

Consil® Bioglass® is a commercially available bioactive glass formulation previously shown in clinical studies to support osteogenesis and the repair of bony defects in dogs and cats. Previous in vitro studies confirm that Consil® particles are able to bond directly with bone while promoting osteoblast proliferation and extracellular matrix production. However, the cellular mechanisms mediating their clinical effect remain unclear. This study evaluated whether enhancement of osteoblast proliferation by Consil® particles is associated with signal transduction. Consil® particles maintained the osteoblast phenotype and enhanced proliferation of canine osteoblasts for up to 21 days in culture. Stimulation of proliferation and maintenance of phenotype expression were accompanied by the modulation of selective cell signaling pathways including integrins, the mitogen-activated protein kinases (MAPKs), and the immediate-early gene c-Jun. These genes have been documented to mediate osteoblast growth and differentiation. The signal transduction occurs in a time-dependent manner in which Consil® particles induce a decrease in the pattern of MAPK and c-Jun gene transcription from 4 to 24 h and a subsequent return to control levels by 7 days in culture. Our observations suggest that Consil® Bioglass® particles may provide cues that enhance cell division necessary for facilitating bone regeneration and the repair of bony defects.

Original languageEnglish (US)
Pages (from-to)380-388
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume94
Issue number2
DOIs
StatePublished - Aug 2010

Fingerprint

Cell signaling
Bioactive glass
Osteoblasts
Glass
Signal transduction
Genes
jun Genes
Bone
Repair
Proteins
Mitogen-Activated Protein Kinases
Defects
Level control
Signal Transduction
Transcription
Cell culture
Phenotype
Bone Regeneration
Immediate-Early Genes
Cells

Keywords

  • Bioactive glass
  • Bone
  • Bone graft
  • Extracellular matrix
  • Osteoblasts

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

Cite this

Consil® bioactive glass particles enhance osteoblast proliferation and selectively modulate cell signaling pathways in vitro. / Au, Angela Y.; Au, Rebecca Y.; Demko, Jennifer L.; McLaughlin, Ron M.; Eves, Barbara E.; Frondoza, Carmelita G.

In: Journal of Biomedical Materials Research - Part A, Vol. 94, No. 2, 08.2010, p. 380-388.

Research output: Contribution to journalArticle

Au, Angela Y. ; Au, Rebecca Y. ; Demko, Jennifer L. ; McLaughlin, Ron M. ; Eves, Barbara E. ; Frondoza, Carmelita G. / Consil® bioactive glass particles enhance osteoblast proliferation and selectively modulate cell signaling pathways in vitro. In: Journal of Biomedical Materials Research - Part A. 2010 ; Vol. 94, No. 2. pp. 380-388.
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