Consensus phosphorylation sites of human GABA(C)/GABA(ρ) receptors are not critical for inhibition by protein kinase C activation

Tadashi Kusama, Kouji Hatama, Manabu Sakurai, Yasuo Kizawa, George R. Uhl, Hajime Murakami

Research output: Contribution to journalArticle

Abstract

The mechanism of inhibition of human GABA(C)/GABA(ρ) receptors by protein kinase C (PKC) activation was investigated in Xenopus oocytes. Phorbol 12-myristate 13 acetate (PMA), a potent PKC activator, at 25 nM inhibited the currents through GABA(ρ2) receptors, which have one consensus phosphorylation site by PKC in the predicted intracellular loops. The time- courses and amplitudes of inhibition were not significantly different from those occurring through GABA(ρ1) receptors, which have six such sites. The inhibitory effect of PMA was also observed after removing each consensus phosphorylation site in both GABA(ρ1) and ρ2 receptors by site-directed mutagenesis. These results suggest that phosphorylation of consensus sites in the intracellular loops is not involved in the inhibition of human GABA(C)/GABA(ρ) receptors by PKC activation.

Original languageEnglish (US)
Pages (from-to)17-20
Number of pages4
JournalNeuroscience Letters
Volume255
Issue number1
DOIs
StatePublished - Oct 9 1998

Fingerprint

gamma-Aminobutyric Acid
Protein Kinase C
Phosphorylation
GABA Receptors
Acetates
Xenopus
Site-Directed Mutagenesis
Oocytes
GABA-C receptor
phorbol-12-myristate

Keywords

  • ρ1 subunit
  • ρ2 subunit
  • GABA(C) receptor
  • Mutagenesis
  • Phosphorylation
  • Protein kinase C
  • Voltage clamp
  • Xenopus oocyte

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Consensus phosphorylation sites of human GABA(C)/GABA(ρ) receptors are not critical for inhibition by protein kinase C activation. / Kusama, Tadashi; Hatama, Kouji; Sakurai, Manabu; Kizawa, Yasuo; Uhl, George R.; Murakami, Hajime.

In: Neuroscience Letters, Vol. 255, No. 1, 09.10.1998, p. 17-20.

Research output: Contribution to journalArticle

Kusama, Tadashi ; Hatama, Kouji ; Sakurai, Manabu ; Kizawa, Yasuo ; Uhl, George R. ; Murakami, Hajime. / Consensus phosphorylation sites of human GABA(C)/GABA(ρ) receptors are not critical for inhibition by protein kinase C activation. In: Neuroscience Letters. 1998 ; Vol. 255, No. 1. pp. 17-20.
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