Connexin43 mRNA contains a functional internal ribosome entry site

Adam Schiavi, Alice Hudder, Rudolf Werner

Research output: Contribution to journalArticlepeer-review

Abstract

A reporter gene construct was used to study the regulation of connexin43 (Cx43) expression, the major gap junction protein found in heart and uterus, in transfected cell lines. The construct had the firefly luciferase gene under the control of the Cx43 promoter. Inclusion of the 5'-untranslated region (UTR) of the mRNA in the construct increased luciferase expression by 70%. A bicistronic vector assay demonstrated that the Cx43 5'-UTR contains a strong internal ribosome entry site (IRES). Deletion analysis localized the IRES element to the upstream portion of the 5'-UTR.

Original languageEnglish (US)
Pages (from-to)118-122
Number of pages5
JournalFEBS Letters
Volume464
Issue number3
DOIs
StatePublished - Dec 31 1999

Keywords

  • Connexin43
  • Internal ribosome entry site
  • Translation control
  • mRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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