Connexin36 is essential for transmission of rod-mediated visual signals in the mammalian retina

Michael R. Deans, Bela Volgyi, Daniel A. Goodenough, Stewart A. Bloomfield, David L. Paul

Research output: Contribution to journalArticle

Abstract

To examine the functions of electrical synapses in the transmission of signals from rod photoreceptors to ganglion cells, we generated connexin36 knockout mice. Reporter expression indicated that connexin36 was present in multiple retinal neurons including rod photoreceptors, cone bipolar cells, and AII amacrine cells. Disruption of electrical synapses between adjacent AIIs and between AIIs and ON cone bipolars was demonstrated by intracellular injection of Neurobiotin. In addition, extracellular recording in the knockout revealed the complete elimination of rod-mediated, on-center responses at the ganglion cell level. These data represent direct proof that electrical synapses are critical for the propagation of rod signals across the mammalian retina, and they demonstrate the existence of multiple rod pathways, each of which is dependent on electrical synapses.

Original languageEnglish (US)
Pages (from-to)703-712
Number of pages10
JournalNeuron
Volume36
Issue number4
DOIs
StatePublished - Nov 14 2002
Externally publishedYes

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Electrical Synapses
Retina
Retinal Rod Photoreceptor Cells
Ganglia
Retinal Neurons
Amacrine Cells
Vertebrate Photoreceptor Cells
Knockout Mice
Injections
connexin 36

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Deans, M. R., Volgyi, B., Goodenough, D. A., Bloomfield, S. A., & Paul, D. L. (2002). Connexin36 is essential for transmission of rod-mediated visual signals in the mammalian retina. Neuron, 36(4), 703-712. https://doi.org/10.1016/S0896-6273(02)01046-2

Connexin36 is essential for transmission of rod-mediated visual signals in the mammalian retina. / Deans, Michael R.; Volgyi, Bela; Goodenough, Daniel A.; Bloomfield, Stewart A.; Paul, David L.

In: Neuron, Vol. 36, No. 4, 14.11.2002, p. 703-712.

Research output: Contribution to journalArticle

Deans, MR, Volgyi, B, Goodenough, DA, Bloomfield, SA & Paul, DL 2002, 'Connexin36 is essential for transmission of rod-mediated visual signals in the mammalian retina', Neuron, vol. 36, no. 4, pp. 703-712. https://doi.org/10.1016/S0896-6273(02)01046-2
Deans, Michael R. ; Volgyi, Bela ; Goodenough, Daniel A. ; Bloomfield, Stewart A. ; Paul, David L. / Connexin36 is essential for transmission of rod-mediated visual signals in the mammalian retina. In: Neuron. 2002 ; Vol. 36, No. 4. pp. 703-712.
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