Connective tissue degrading enzymes of human leukocytes

A. L. Oronsky, R. J. Perper

Research output: Contribution to journalArticle

Abstract

Human leukocytes, when exposed to aggregated human γ globulin (AHGG) or immune complexes (isolated from RA synovial fluid) fixed to a cartilagenous surface, release neutral proteases that degrade the extracellular matrix of cartilage. The chondromucoprotein destruction by these proteases is suppressed by a variety of synovial fluids but is not susceptible to inhibition by trypsin, chymotrypsin, elastase inhibitors, or a combination of these agents. The inhibitory effect of synovial fluid can be reversed in the presence of increasing enzyme concentrations. Intact viable human polymorphonuclear leukocytes in the presence of AHGG also release a collagenase precursor that can be activated by limited proteolysis with trypsin or RA synovial fluids. Enzyme release (neutral proteases) by phagocytosing cells is inhibited by the inflammatory agents phenylbutazone and colchicine; these agents do not affect release of the collagenase precursor. However, the latent collagenase release is susceptible to inhibition when leukocytes are preincubated (prior to exposure to AHGG) with inhibitors of protein synthesis. Under these conditions, neutral protease release is unaffected.

Original languageEnglish (US)
Pages (from-to)233-253
Number of pages21
JournalAnnals of the New York Academy of Sciences
VolumeVol. 256
StatePublished - 1975
Externally publishedYes

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Synovial Fluid
Connective Tissue
Leukocytes
Collagenases
Peptide Hydrolases
Tissue
Fluids
Enzymes
Proteolysis
Cytophagocytosis
Phenylbutazone
Protein Synthesis Inhibitors
Pancreatic Elastase
Globulins
Colchicine
Cartilage
Antigen-Antibody Complex
Trypsin
Extracellular Matrix
Neutrophils

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Connective tissue degrading enzymes of human leukocytes. / Oronsky, A. L.; Perper, R. J.

In: Annals of the New York Academy of Sciences, Vol. Vol. 256, 1975, p. 233-253.

Research output: Contribution to journalArticle

Oronsky, A. L. ; Perper, R. J. / Connective tissue degrading enzymes of human leukocytes. In: Annals of the New York Academy of Sciences. 1975 ; Vol. Vol. 256. pp. 233-253.
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